A kind of pyranolactone compound and its preparation method and application
A technology of pyranolide and compound, applied in the field of phytochemistry, can solve the problem that the components have not been identified, and achieve the effects of improving the smoking quality of cigarettes, significantly antibacterial activity, and improving the antibacterial function
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Preparation of pyranolactone compound C 17 h 20 o 4 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:
[0048] 1. Extraction of extract: get the tobacco stem bark and pulverize it, use high-concentration methanol (wt%: 95%) or high-concentration ethanol (wt%: 95%) or high-concentration acetone (wt%: 70%) as the extraction solvent, extract Solvent: Tobacco stem bark (weight ratio) = 5:2, soak for 54 hours, extract 4 times, combine extracts, filter and concentrate to obtain extract.
[0049] 2. Silica gel column chromatography: dissolve the extract obtained in step 1 with 2.5 times the weight ratio of pure methanol or pure ethanol or pure acetone, then mix the sample with 1.2 times the weight ratio of 80-100 mesh silica gel, and use 3 times the weight ratio Amount of 250 mesh silica gel dry-packed column for silica gel column chromatography; the volume ratio i...
Embodiment 2
[0054] Tobacco stem bark samples come from Yuxi, Yunnan, and the variety is Yuxi Honghua Dajinyuan. Sampling 2.5 kg of tobacco stem bark and pulverizing it, extracting 5 times with 95 wt % methanol aqueous solution for 24 hours each time, combining the extracts, filtering, and concentrating under reduced pressure to obtain an extract to obtain 115 g of extract. After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 120g of 100-mesh silica gel, pack 0.6kg of 160-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging parts with the same polarity to obtain 8 parts, wherein the volume ratio is The 8:2 chloroform-acetone eluted part was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 62% methanol as the mobile phase, Zorbax SB-C18 (21.2×250mm, 5μm) prepar...
Embodiment 3
[0056] The tobacco stem bark sample was from Dali, Yunnan, and the variety was Yunyan 300. The tobacco stem bark was sampled 3.5kg and chopped, extracted 4 times with 95wt% ethanol aqueous solution for 48 hours each time, the extracts were combined, filtered, and concentrated under reduced pressure to obtain Ointment, get extractum 255g. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 250 g of 80 mesh thick silica gel, and 1.2 kg of 200 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging fractions of the same polarity to obtain 8 fractions, of which the volume ratio is 8 The chloroform-acetone elution part of :2 is separated by Agilent 1100 semi-preparative high performance liquid chromatography, with 58% methyl alcohol as mobile phase, Zorbax SB-C18 (21.2 * 250mm, 5 μ m) preparative column i...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com