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Application of histone methyltransferase EZH2 in preparation of biomarker for diagnosing sepsis

A technology of methyltransferase and sepsis, applied in the field of bioengineering, can solve the problems that cannot be used for sepsis treatment, lack of sensitivity and specificity, etc.

Inactive Publication Date: 2020-07-14
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biomarkers that have received widespread clinical attention include C-reactive protein (CRP), procalcitonin (PCT), blood lactate, IL-6, leukocyte DR antigen (HLA-DR), polysaccharide-binding protein (LBP), myeloid Cell triggering receptor (TREM-1) and soluble urokinase plasminogen activator receptor (suPAR), etc., but all have certain limitations, lack of sensitivity and specificity, and cannot be used for sepsis treatment potential target

Method used

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  • Application of histone methyltransferase EZH2 in preparation of biomarker for diagnosing sepsis
  • Application of histone methyltransferase EZH2 in preparation of biomarker for diagnosing sepsis
  • Application of histone methyltransferase EZH2 in preparation of biomarker for diagnosing sepsis

Examples

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Effect test

Embodiment 1

[0021] Example 1 Comparison of EZH2 expression changes on T lymphocytes between sepsis and healthy control groups

[0022] Compared with healthy controls, the positive rate and mean fluorescence intensity of EZH2 expression in lymphocytes in patients with sepsis were significantly increased (eg figure 1 ). Compared with the healthy control group, EZH2 in peripheral blood CD4 of patients on day 1, 3 and 7 + The positive rates on lymphocytes were significantly increased (P<0.001); the expression intensity was also significantly increased (P<0.001).

[0023] We further detected and analyzed the effect of EZH2 on CD8 in patients with sepsis. + The expression on lymphocytes showed that both the positive rate and the average fluorescence intensity were significantly higher than those in the control group. Further analysis of pairwise comparisons on days 1, 3, and 7 in the sepsis patient group showed that the positive rate of EZH2 on CD4+ T lymphocytes on day 7 was significantly h...

Embodiment 328

[0025] The independent risk factor analysis of embodiment 328 days death

[0026] Univariate logistic regression analysis was performed according to the indicators with statistically significant differences between the death group and the survival group in the sepsis group, and multivariate logistic regression analysis was performed for indicators with statistical significance in the single factor, and participants in the multivariate regression analysis were analyzed. index. Combined with the test results, the independent risk factors for 28-day death were the use of ventilator (OR: 4.107 (1.168-14.436). p = 0.028), APACHE II (OR: 1.440 (1.159-1.791 ), p=0.001), SOFA (OR: 1.495 (1.168-1.915), p=0.001), CD8 + The percentage of T lymphocytes (%) (OR: 1.114 (1.008-1.231), p = 0.034), CD8 + T lymphocyte EZH2 + (OR: 1.022 (1.002-1.043), p=0.032); (as shown in Table 3.1), the indicators were included in the ROC curve analysis to evaluate the predictive performance related to 28-...

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Abstract

The invention provides an application of histone methyltransferase EZH2 in preparation of a biomarker for diagnosing sepsis severity or prognosis. The invention also provides a kit for diagnosing sepsis severity or prognosis. The kit contains a reagent for detecting histone methyltransferase EZH2 in a body fluid sample. The invention finds that the expression quantity of EZH2 in peripheral blood Tcells of a clinical sepsis patient is obviously higher than that of EZH2 in a healthy examinee; the SOFA score of the sepsis patient is positively correlated; through ROC curve analysis, it is foundthat the expression level of EZH2 in T cells can be used as a new biomarker for predicting sepsis prognosis, EZH2 is provided as an index for diagnosis, prognosis and treatment monitoring of sepsis infection, and the severity of sepsis can be evaluated.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a biomarker, specifically the use of histone methyltransferase EZH2 in preparing a biomarker for diagnosing sepsis. Background technique [0002] Sepsis is a systemic inflammatory response syndrome caused by infectious factors accompanied by acute organ dysfunction. Some patients with sepsis who are still accompanied by uncorrectable persistent hypotension after adequate fluid resuscitation will further develop into septic shock. With the aging of the population and the increase of invasive medical methods, the incidence of sepsis continues to rise. Every year, millions of sepsis patients are added worldwide, of which more than 1 / 4 of the patients die. One, and the cost of treatment is expensive (more than 80,000 yuan per case in my country on average), which has caused a heavy economic burden on patients, families and society, so early diagnosis and treatment of sepsis and its complic...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N21/64
CPCG01N33/573G01N33/56972G01N21/6428G01N2800/26G01N2800/52
Inventor 唐伦先孙宏包晓玮赵冬旸刘娜周如女许明正李珂吕迪宇白建文
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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