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A kind of composite quantum dot coded microsphere based on natural spiny pollen and its preparation method

A composite quantum and dot coding technology is applied in the field of composite quantum dot coding microspheres based on natural prickly pollen and its preparation, which can solve the problems of complex preparation, simple structure and insufficient coding quantity, and achieves wide excitation spectrum and quantity Huge, good coding stability

Active Publication Date: 2020-11-24
NANJING DRUM TOWER HOSPITAL
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  • Description
  • Claims
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a kind of composite quantum dot coded microsphere based on natural spiny pollen and its preparation method for the deficiencies of the above-mentioned prior art. The method uses natural spiny pollen as the encoding carrier. Quantum dots are modified layer by layer on it, and microspheres with unique morphology and multiple coding combinations are prepared, which solves the shortcomings of traditional coded microspheres, such as simple structure, complicated preparation, and insufficient coding quantity

Method used

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  • A kind of composite quantum dot coded microsphere based on natural spiny pollen and its preparation method
  • A kind of composite quantum dot coded microsphere based on natural spiny pollen and its preparation method
  • A kind of composite quantum dot coded microsphere based on natural spiny pollen and its preparation method

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preparation example Construction

[0039] The present invention provides the preparation method of the composite quantum dot coded microsphere based on natural spiny pollen, technological process is as follows figure 1 shown, including the following steps:

[0040] (1) Soak the spiny pollen in absolute ethanol and shake to obtain a pollen dispersion in which the pollen grains are fully peeled off, then dry the pollen dispersion to disperse and screen the dried pollen grains with distinct particles, and place the dried pollen grains in Calcination at a high temperature of 300°C and continuous flow of nitrogen for 12 hours to completely carbonize the fluorescent organic substance to eliminate autofluorescence;

[0041] (2) Soak the pollen substrate in the negative polyelectrolyte solution and the positive polyelectrolyte solution in turn to obtain a positively charged microsphere substrate, and use the layer-by-layer deposition technology of polyelectrolyte to coat the outer surface of the positively charged micr...

Embodiment 1

[0055] A kind of sunflower pollen-based composite quantum dot coded microsphere, prepared by the following steps:

[0056] (1) Carbonization treatment to prepare sunflower pollen substrate after eliminating autofluorescence

[0057] Soak sunflower pollen in absolute ethanol and keep shaking for 20 minutes to obtain a dispersion in which the pollen grains are fully peeled off. Put the dispersion on a hot table at 100°C and heat until the ethanol is completely volatilized. Sunflower pollen grains: put the dried sunflower pollen grains in a muffle furnace, calcinate them at a high temperature of 300°C, and continue feeding nitrogen gas for 12 hours to obtain a positively charged sunflower pollen substrate after the fluorescent organic substances are completely carbonized, such as figure 1 shown;

[0058] Fluorescent characterization of natural sunflower pollen and sunflower pollen substrates after carbonization using laser scanning confocal fluorescence microscopy, figure 2 It i...

Embodiment 2

[0063] A kind of composite quantum dot coded microsphere based on ragweed pollen is prepared through the following steps:

[0064] (1) Carbonization treatment to prepare ragweed pollen substrate after eliminating autofluorescence

[0065] Soak ragweed pollen in absolute ethanol and keep shaking for 20 minutes to obtain a dispersion in which the pollen grains are fully peeled off. Put the dispersion on a hot stage at 100°C and heat until the ethanol is completely volatilized. Dried ragweed pollen grains; place the dried ragweed pollen grains in a muffle furnace, calcinate them at a high temperature of 300°C, and continue feeding nitrogen gas for 12 hours to obtain a positively charged ragweed pollen base after the fluorescent organic substances are completely carbonized;

[0066] (2) Preparation of composite quantum dot-encoded microspheres based on ragweed pollen by polyelectrolyte layer-by-layer adsorption method

[0067] Dissolve PAH and PAA solid particles in 0.5mol / L NaCl...

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Abstract

The invention discloses a composite quantum dot encoding microsphere based on natural spiny pollen and a preparation method thereof, the preparation method takes the natural spiny pollen as an encoding substrate, and quantum dots with different characteristic emission peak positions and intensities are modified on the surface layer by layer; after autofluorescence of the pollen substrate is eliminated through carbonization treatment, the porous and thorn-shaped surface structure provides high porosity and a large specific surface area, the adsorption amount of target molecules can be increased, then a detection fluorescence signal is remarkably enhanced, and the detection sensitivity is improved; a polyelectrolyte layer-by-layer deposition technology is adopted, so that quantum dots with different layer numbers, different types or different sizes are adsorbed on the outer surface of the pollen substrate layer by layer, the method is simple and easy to implement, various types of quantum dots can be uniformly combined, and the coding stability is good; the coding form of multiple characteristic emission peaks and intensities can be realized by adopting the combination of different types and different layer numbers of sub-points, the coding quantity is expanded, and meanwhile, the fluorescence coding information of the coded microspheres can be accurately controlled, so that therequirements of multi-index and high-flux biological detection are met.

Description

technical field [0001] The invention relates to the technical field of biomaterials, and relates to a quantum dot encoded microsphere using natural spiny pollen as a coding base and a preparation method thereof, in particular to a composite quantum dot encoded microsphere based on natural spiny pollen and its Preparation. Background technique [0002] In order to study a large number of intermolecular interactions, such as the interaction between proteins and nucleic acids, between proteins and proteins, and between proteins and drugs, high-throughput test platforms have emerged one after another, and high-throughput test platforms require high-throughput Molecular carriers, so-called coding carriers, are used to identify different molecules and the interactions between molecules, and then analyze the results by reading the corresponding coded information. Among the common coding carriers, the array biochip uses the fixed positions of molecules, that is, the coordinates, as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/88C09K11/56
CPCC09K11/02C09K11/025C09K11/565C09K11/883
Inventor 王月桐商珞然赵远锦
Owner NANJING DRUM TOWER HOSPITAL
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