Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Efficient starting culture medium for directly inducing tetraploid paulownia large-field stem segment to re-grow adventitious buds, and application

A high-efficiency start-up and culture medium technology, applied in the biological field, can solve the problems of loss of excellent plant traits, reduction of reproduction coefficient, vitrification, etc., to achieve the effect of saving manpower and high reproduction coefficient

Active Publication Date: 2020-07-14
SHANDONG FOREST SCI RES INST +2
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In the process of plant tissue culture, the explants are prone to mutation, poor growth or vitrification in the process of subculture due to the long-term exposure to high-concentration hormone induction, ultraviolet radiation (sterilization), and formaldehyde sterilization in the culture room. effect, resulting in the loss of good traits of the propagated plants, or a reduction in the reproduction coefficient
However, there are no related studies using the organs or tissues of tetraploid paulownia in field as explants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient starting culture medium for directly inducing tetraploid paulownia large-field stem segment to re-grow adventitious buds, and application
  • Efficient starting culture medium for directly inducing tetraploid paulownia large-field stem segment to re-grow adventitious buds, and application
  • Efficient starting culture medium for directly inducing tetraploid paulownia large-field stem segment to re-grow adventitious buds, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Cut off the leaves of the tetraploid paulownia branches retrieved from the field, rinse the branches with tap water first, and then cut them into stems with 1 bud. Generally, the length of the stem is about 1.5 cm. After the disinfection treatment, inoculate on the stem initiation medium, the composition of the stem initiation medium is MS+6-BA 0.5mg / L+GA 0.05mg / L+KT 0.05mg / L+NAA0.05mg / L+agar 4.5 g / L+ sucrose 30g / L (pH value 5.8), culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After culturing for about 20 days, the average proliferation coefficient reached 3.8. See figure 1 .

Embodiment 2

[0024] Cut off the leaves of the tetraploid paulownia branches retrieved from the field, rinse the branches with tap water first, and then cut them into stems with 1 bud. Generally, the length of the stem is about 1.5 cm. After the disinfection treatment, it was inoculated on the starting medium of the stem segment, and the composition of the starting medium of the stem segment was MS+6-BA4.0mg / L+GA1.0mg / L+KT 0.5mg / L+NAA1.0mg / L+agar 5.0 g / L + sucrose 40g / L (pH value 5.8), culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After culturing for 20 days, the average proliferation coefficient reached 6.3. See figure 2 .

Embodiment 3

[0026] Cut off the leaves of the tetraploid paulownia branches retrieved from the field, rinse the branches with tap water first, and then cut them into stems with 1 bud. Generally, the length of the stem is about 1.5 cm. After the disinfection treatment, inoculate on the stem initiation medium, the composition of the stem initiation medium is MS+6-BA 14.0mg / L+GA 3.0mg / L+KT 3.0mg / L+NAA3.0mg / L+agar 6.0 g / L+sucrose 50g / L (pH 6.0), the culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After culturing for 20 days, the average proliferation coefficient reached 4.7. See image 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an efficient starting culture medium for directly inducing a tetraploid paulownia large-field stem segment to re-grow adventitious buds, and an application. The large-field reclaimed tetraploid paulownia branches are cut into a stem segment with buds, and after being strictly sterilized, the stem segment is inoculated into a stem segment starting culture medium, wherein thestem segment starting culture medium is composed of an MS culture medium added with 0.5-14.0 mg / L of 6-benzylamino adenine, 0.05-3.0 mg / L of gibberellins, 0.05-3.0 mg / L of kinetin, 0.05-3.0 mg / L of naphthylacetic acid, 4.0-6.0 g / L of agar and 30-50g / L of saccharose, wherein the culture condition is that the temperature in the day time is 25 DEG C, the temperature at night is 18 DEG C, the illumination intensity is 2500 Lx, and the illumination time is 13 h / d. After culture of 20 days, adventitious buds are induced, and the proliferation coefficient reaches 7.6 on average. Therefore, manpower, material resources and financial resources are saved, and relatively high propagation coefficient is obtained.

Description

technical field [0001] The invention relates to a high-efficiency starting medium for directly inducing adventitious bud regeneration from tetraploid paulownia field stems and its application, belonging to the field of biotechnology. Background technique [0002] Paulownia is a plant of the genus Paulownia in the family Scrophulariaceae. It is native to my country. It has no flying catkins, beautiful trees, and a long history of cultivation. It is naturally distributed in 25 provinces, municipalities, and autonomous regions in my country. It is an important fast-growing timber and native tree species for landscaping in my country. The leaves, flowers, fruits and bark of Paulownia can be used as medicine, and the leaves and flowers of Paulownia can be used as feed. Paulownia wood is high-quality and high-grade, corrosion-resistant, acid and alkali resistant, wear-resistant, easy to process, easy to carve, long fiber, light color, easy to dye, fine texture, natural color, beau...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 毛秀红刘泉刘欣闫少波毛欣王迎仲伟国亓玉昆朱文成梁燕赵青松仲凤维
Owner SHANDONG FOREST SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com