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Key gene of methylation of side chains of corn xylan, expression vector and application

A technology of key genes and expression vectors, applied in the field of expression vectors and applications, key genes of corn xylan side chain methylation, can solve problems such as unclear mechanism of action, achieve the goal of reducing production risks and improving drought resistance Effect

Active Publication Date: 2020-07-10
HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS
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AI Technical Summary

Problems solved by technology

Genes related to cell wall synthesis play a key role in the process of plant drought resistance, and xylan is an important part of the cell wall. There are still many genes related to xylan synthesis that have not been discovered, and the role of drought resistance is regulated by regulating the shape of the cell wall Mechanism is not very clear

Method used

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  • Key gene of methylation of side chains of corn xylan, expression vector and application
  • Key gene of methylation of side chains of corn xylan, expression vector and application
  • Key gene of methylation of side chains of corn xylan, expression vector and application

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Embodiment

[0045] 1. Discovery and phenotypic identification of wi6 mutants

[0046] Our research group crossed the inbred line containing the MU transposon with Zong 31 in the early stage. In the F2 segregation generation, it was found that under normal conditions or drought stress, a kind of maize plants at the 5-leaf stage showed wilting symptoms. Growth and development, the phenotype is more and more obvious, mainly on the new leaves, the symptoms are mild or normal, and the wi6 mutant is named; compared with the wild type, the mutant shows short plants, new leaves wilting and curly, The leaf color turns green, and when the leaf loses water seriously, it will gradually die from the leaf tip ( figure 1 ). The mutant may be affected by temperature. Under high temperature (noon) conditions, even if there is sufficient water, wi6 will show wilting traits. In rainy days, morning or evening, the mutant's wilting symptoms will be alleviated.

[0047] 2. Identification of physiological ind...

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Abstract

The invention belongs to the field of genetic breeding of crops, and relates to a key gene of methylation of side chains of corn xylan, an expression vector and application. According to the key geneof methylation of the side chains of the corn xylan, the expression vector and application, with the drought-stress-sensitive character as an entry point, the xylan synthesis key gene ZmGXM1 is obtained through a classical map-based cloning technology, by means of a characteristic mutant, a characteristic wild-type material and a novel molecular biological technology, the action mechanism of the xylan synthesis key gene of regulating and controlling the drought-resistant capability by adjusting the morphology of cell walls is analyzed, and a novel direction and study material are provided forstudies of genes related to xylan synthesis, the forming mechanism of cell walls and drought-resistant breeding.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a key gene, expression vector and application of corn xylan side chain methylation. Background technique [0002] In dicotyledonous plants (Arabidopsis thaliana as an example), the research on the key genes in the xylan synthesis pathway is relatively clear, but the synthesis and methylation of xylan side chains have not been clearly understood. In maize, most of the genes related to xylan synthesis are homologous to dicotyledonous plants, but there are still great differences in the synthesis mechanism and gene function, especially the methylation related genes of the side chain after xylan synthesis It has not been discovered, and its mechanism of action in xylan synthesis is still unclear. In maize breeding, improving the drought resistance of maize is an important means to achieve high and stable maize yield. Genes related to cell wall synthesis play a key role in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261C12N15/8273
Inventor 曹丽茹鲁晓民张前进郭书磊张新王振华魏昕魏良明
Owner HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS
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