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Bacillus velezensis BV03 inoculant and preparation method and application thereof

A technology of Bacillus and Velez, applied in the field of Bacillus Velez BV03 inoculum and its preparation, can solve the problems of functional limitation and weakening of efficacy, and achieve the effects of easy operation, simple preparation method, and promotion of plant growth

Active Publication Date: 2020-07-10
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the differences in the adaptability, culture and / or storage conditions of different strains in the environment, the functions of each strain in the compound bacterial agent are often limited, and the efficacy is weakened after application.

Method used

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  • Bacillus velezensis BV03 inoculant and preparation method and application thereof
  • Bacillus velezensis BV03 inoculant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of Bacillus Bacillus BV03 bacterial agent of embodiment 1

[0028] A bacterium agent of Bacillus Velez BV03, which is a wettable powder containing Bacillus Velez BV03, and Bacillus Velez BV03 has been preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC). The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18936, the preservation date is November 11, 2019, and the classification name is Bacillus velezensis;

[0029] The preparation method of bacterial agent comprises the steps:

[0030] (1) Inoculate the preserved Bacillus Bacillus BV03 strain into liquid LB medium, and cultivate it at 30°C ± 1°C for 16 hours or to the logarithmic growth phase to obtain seed liquid for inoculation. Below 1.0×10 8 cfu / mL;

[0031] (2) Inoculate the seed liquid into the fermentation medium with an inoculum of 10-12% (volume percentage) for cul...

Embodiment 2

[0038] The growth-promoting action of embodiment 2 Bacillus Bacillus BV03 inoculum in capsicum industrialized seedling cultivation

[0039] The treatment group is to add the Bacillus Bacillus BV03 inoculum prepared in Example 1 into the seedling cultivation substrate at 10g / L, 5g / L, 2g / L, and obtain the cultivation substrate I, II, and III after fully mixing, according to Conventional method of sowing and routine management of Zhongjiao No. 6,

[0040] The cultivation group Zhongjiao No. 6, which directly used the seedling substrate as the cultivation substrate, was used as the negative control.

[0041] With the addition of nitrogen, phosphorus and potassium compound fertilizers (nitrogen: phosphorus: potassium = 15:15:15) in the cultivation medium, the cultivation group Zhongjiao No. 6 was used as a positive control (the amount used was 60g / m 2 ),

[0042] Each group treated 36 strains and repeated 3 times.

[0043] After 21 days, observe and count the plant height, stem ...

Embodiment 3

[0047] Example 3 The effect of the Bacillus velei BV03 inoculum on the content of available nitrogen, available phosphorus and available potassium in the cultivation substrate in the industrialized seedling cultivation of pepper

[0048] Example 2 After 22 days of sowing, the cultivation substrates I, II, III of the treatment group and the cultivation substrate of the negative control group were tested for available nitrogen, available phosphorus and available potassium content, and the detection method was referred to "Soil Agrochemical Analysis and Environmental Monitoring" (Edited by Yang Jianhong, Wang Chenglin, Dai Henglin, China Dadi Publishing House, 2008 edition), the detection of available nitrogen adopts alkali solution diffusion method, the detection of available phosphorus adopts 0.5M sodium bicarbonate leaching-molybdenum antimony anti-colorimetric method and available potassium detection Using NH 4 OAc Leaching - Flame Photometry. The test results are shown in T...

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Abstract

The invention relates to the technical field of microorganism application, in particular to a bacillus velezensis BV03 inoculant, a preparation method thereof and application thereof in growth of solanaceae plants and / or cucurbitaceae plants. The bacillus velezensis BV03 is already preserved in China General Microbiological Culture Collection Center, the preservation address is No.3, Yard 1, Beichen west road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18936, and the preservation date is November 11, 2019, and the bacillus velezensis is classified and named as bacillus velezensis. The preparation method comprises the following steps: acquiring a seed liquid, fermenting the seed liquid to obtain a fermentation liquid pre-preparation, and preparing the inoculant. The growth promoting effect of the inoculant in industrial seedling culture reaches 30%, the seedling strengthening index is increased by more than 45%, the yield increasing effects are 48% and 34% respectively when the inoculant is used in transplanting, and the fruiting rate is more than 85%.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a Bacillus velei BV03 bacterial agent and a preparation method and application thereof. Background technique [0002] Since the 21st century, with the rapid economic growth, population growth and increasing attention to food health, the agricultural production area has continued to expand, and the output has also increased day by day, and has achieved considerable results. However, in the process of development, various problems have become increasingly prominent. In order to ensure the growth of agricultural products, especially vegetables and fruits, farmers pursue economic benefits and increase yield per mu, and it is not uncommon for farmers to use chemical fertilizers irregularly or exceed the standards. These behaviors endanger agricultural products, increase the cost of agricultural production, and reduce the value of agricultural products. In addition, the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P21/00A01G22/05A01G7/06C12R1/07
CPCC12N1/20A01G22/05A01G7/06Y02A40/10
Inventor 谢雪迎张新建周方园周红姿张广志赵晓燕吴晓青范素素王加宁
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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