Preparation method of HA (hyaluronic acid) blocking agent and antibody detection system

Abstract

The invention belongs to the technical field of biomedicine, and discloses a preparation method of an HA (hyaluronic acid) blocking agent and an antibody detection system. The preparation method comprises the following steps of purifying an antibody by ammonium sulfate with concentration of 33%-50%; adding glutaraldehyde into the obtained purified antibody, and crosslinking; after dialysis is completed, recycling the antibody, and storing; mixing a sample, a detection microsphere, a heterotropic antibody interference indication microsphere and a second antibody for marking a detectable signal,so as to form a reaction system; and detecting the detectable signal on the microsphere in a second antibody-antigen-first antibody-microsphere quaternary compound in the reaction system, comparing with a standard value or a standard curve, and determining whether a target antigen exists and / or the number of target antigens in the reaction system. The prepared HA blocking agent has the advantagesthat the anti-interference ability is strong, the HA interference is completely eliminated, the interference to immunity detection is avoided, the occurrence rate of false positive is effectively reduced, and the accuracy and precision rate of immunity detection are improved; the preparation method is simple, the control is easy, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of an HA blocker and an antibody detection system. Background technique [0002] At present, the closest existing technology: blockers, also called receptor antagonists, refer to a class of ligand substances that can bind to receptors and prevent the effects of agonists. Antagonists have affinity for the corresponding receptor, but have no potency, thereby inhibiting the action of the agonist on the receptor. Antagonists can bind to the active site of a receptor, or to an allosteric site, or even to a site that usually has no biological regulation alone to achieve an effect. Antagonists and receptors have different binding properties, so the binding time can be long or short, and the reversibility of binding is also different. Antagonists are classified into competitive antagonists and non-competitive antagonists. Most drug antagonists comp...

AI Technical Summary

Problems solved by technology

The prepared antibody has poor affinity, and the process is complicated, and the process parameters are difficult to control accurately

[0005] In summary, the existing problems in the prior art are: the existing HA blockers have low affinity and cannot completely eliminate HA interference, which will affect the results of immunoassays; the preparation process of HA blockers is complex, Difficult to promote

[0006] Difficulty in solving the above technical problems: At present, there are commercialized HA blockers on the market such as: IIR, NS-ABT, Heteroblock, MAB33 and polyMAB33, which have poor affinity and are not effective in reducing interference when combined with HA. Significantly, leading to wrong results; in addition, the preparation method of the existing HA blocking agent is complicated, not suitable for large-scale production, and difficult to popularize and use

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