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A kind of culture method of ammonia oxidizing bacteria

A technique of ammonia oxidizing bacteria and a cultivation method, which is applied in the field of cultivating ammonia oxidizing bacteria, can solve the problems of multiple components, secondary pollution, and containing metal salts in the composition, and achieve the effects of high activity, promoting activity, and increasing growth rate

Active Publication Date: 2022-07-08
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of this method has many ingredients and contains metal salts, which will cause secondary pollution if used for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The cultivation of ammonia oxidizing bacteria is carried out in a 100L aeration reactor. The aeration reactor is also equipped with stirring, pH and dissolved oxygen monitoring, and automatic feeding system. During the cultivation, the temperature was set at 30°C-35°C, and the pH was set at 7.5-8.0.

[0025] (1) First, add 50L of culture solution into the aeration reactor, open the aeration system without inoculating microorganisms and adjust the aeration amount for aeration until the DO concentration no longer increases, thereby determining the DO concentration of the culture system. The upper limit DOmax (8.8mg / L) and the corresponding aeration volume were maintained during the whole culture process. The microbial culture solution contains: 50 g of ammonium sulfate (equivalent to about 212 mg / L of ammonia nitrogen concentration), 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfate heptahydrate, and 7 g of potassium dihydrogen phosphate.

[0...

Embodiment 2

[0030] The cultivation of ammonia oxidizing bacteria is carried out in a 100L aeration reactor. The aeration reactor is also equipped with stirring, pH and dissolved oxygen monitoring, and automatic feeding system. During the cultivation, the temperature was set at 30°C-35°C, and the pH was set at 7.8-8.2.

[0031] (1) First, add 50L of culture solution into the aeration reactor, open the aeration system without inoculating microorganisms and adjust the aeration amount for aeration until the DO concentration no longer increases, thereby determining the DO concentration of the culture system. The upper limit DOmax (8.5mg / L), and the corresponding aeration volume, and the ventilation volume remained unchanged throughout the culture process. The microbial culture solution contains: 120 g of ammonium sulfate (equivalent to about 500 mg / L of ammonia nitrogen concentration), 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfate heptahydrate, and 7 g of potass...

Embodiment 3

[0036] The cultivation of ammonia oxidizing bacteria is carried out in a 100L aeration reactor. The aeration reactor is also equipped with stirring, pH and dissolved oxygen monitoring, and automatic feeding system. During the cultivation, the temperature was set at 30°C-35°C, and the pH was set at 7.8-8.2.

[0037] (1) First, add 50L of culture solution into the aeration reactor, open the aeration system without inoculating microorganisms and adjust the aeration amount for aeration until the DO concentration no longer increases, thereby determining the DO concentration of the culture system. The upper limit DOmax (8.9mg / L) and the corresponding aeration volume were maintained during the whole culture process. The microorganism culture solution contains: 50 g of ammonium sulfate, 1.5 g of ferrous sulfate, 0.5 g of calcium chloride, 7 g of magnesium sulfate heptahydrate, and 7 g of potassium dihydrogen phosphate.

[0038] (2) Inoculate ammonia oxidizing bacteria (nitrosation ra...

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PUM

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Abstract

The invention relates to a method for culturing ammonia oxidizing bacteria. First, the upper limit value DOmax of the DO concentration of the culture system and the corresponding aeration amount are determined; after inoculating the microorganisms, the optimum growth state period of the microorganisms is determined, and the optimum growth state of the microorganisms is regulated by the concentration of the microorganisms. The dissolved oxygen concentration in the optimal growth state period is not less than 5% DOmax; when the DO rises to 30%-70% DOmax, the feeding system is started, and when the DO drops by 10-50 percentage points, the feeding is stopped; this cycle continues until the culture Finish. The ammonia oxidizing bacteria obtained by the invention has high activity, can realize rapid growth and reproduction, and solves the problem of slow growth of bacterial sources required for industrial application.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and particularly relates to a method for culturing ammonia oxidizing bacteria. Background technique [0002] Nitrifying bacteria, as the main functional microorganisms for the removal of ammonia nitrogen pollutants, belong to the chemoautotrophic type, including ammonia oxidizing bacteria and nitrite oxidizing bacteria. Among them, ammonia oxidizing bacteria are responsible for oxidizing ammonia to nitrite, and nitrite oxidizing bacteria are responsible for further oxidizing nitrite to nitrate. Nitrifying bacteria grow slowly, have a long generation time, and have a slow growth rate. Especially with the development of some new biological denitrification processes such as short-range nitrification and denitrification and short-range nitrification anammox, how to control the ammonia oxidation process in the nitrite stage is very important, and the rapid culture and reproduction of ammon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20Y02W10/10
Inventor 高会杰孙丹凤陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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