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Method for preparing dental pulp mesenchymal stem cells

A technology of dental pulp stem cells and mesenchymal stem cells, applied in cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve the problems of small deciduous teeth, pulp extraction, low efficiency, etc., and achieve high extraction success rate , convenient operation, and the effect of saving raw materials

Pending Publication Date: 2020-06-26
上海锐赛循益生物技术有限公司
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Problems solved by technology

[0003] Because the pulp tissue is hidden in the pulp cavity, and the deciduous teeth are small in size, it is inconvenient to cut and expose the pulp cavity. Therefore, in the prior art, a pulp extraction needle is usually used to remove the pulp from the pulp cavity. However, this method is not only efficient Too low and the pulp cannot be completely removed, resulting in waste of deciduous raw materials

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  • Method for preparing dental pulp mesenchymal stem cells
  • Method for preparing dental pulp mesenchymal stem cells
  • Method for preparing dental pulp mesenchymal stem cells

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Embodiment Construction

[0013] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0014] Such as figure 1 As shown, this embodiment provides a method for preparing dental pulp mesenchymal stem cells, comprising the following steps:

[0015] S1 the step of collecting deciduous tooth tissue;

[0016] S2 the step of transporting the collected deciduous tooth tissue to the laboratory under low temperature conditions;

[001...

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Abstract

The invention discloses a method for preparing dental pulp mesenchymal stem cells. The method includes the following steps of S1, collecting deciduous tooth tissues; S2, transferring the collected deciduous tooth tissues to a laboratory under low temperature conditions; S3, using hydraulic forceps to squeezing and crushing the deciduous tooth tissues; S4, separating dental pulp from the broken deciduous tooth tissues; S5, putting the separated dental pulp into a laboratory small masher for mashing treatment; and S6, performing amplified culturing on dental pulp stem cells. According to the method for preparing the dental pulp mesenchymal stem cells, the hydraulic forceps are adopted to crush the deciduous tooth tissues, the deciduous tooth tissues are squeezed and crushed, and the dental pulp is mashed, and therefore the operation is convenient, the efficiency is high, the raw materials are saved, and the extraction success rate of the stem cells is high.

Description

technical field [0001] The invention relates to the technical field of cell life science, in particular to a method for preparing dental pulp mesenchymal stem cells. Background technique [0002] Pulp tissue is located in the pulp cavity inside the tooth and is the only soft tissue in the tooth tissue. Through the study of human dental pulp cells, a cell with a very similar immune phenotype and the ability to form mineralized nodules with bone marrow mesenchymal stem cells was found. The cells were spindle-shaped in shape, capable of self-renewal and multidirectional differentiation , has a strong cloning ability. These fibroblast cells isolated from dental pulp tissue are called dental pulp stem cells (Dental Pulp Stem Cells, DPSCs). Dental pulp stem cells have the potential of multi-directional differentiation. In addition to cells capable of forming mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelial, liver, nerve and...

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0664C12N2509/00
Inventor 钟军朱建斌
Owner 上海锐赛循益生物技术有限公司
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