Totally-enclosed cell culture system

Abstract

The invention provides a totally-enclosed cell culture system. The totally-enclosed cell culture system at least comprises the following parts: an incubator, a culture tank, an airflow assembly, a liquid flow assembly, a temperature adjusting assembly and a central controller. The system provided by the invention realizes a constant-temperature culture environment, adopts a perfusion manner to carry out cell culture and realizes a totally-enclosed integrated process from cell activation, infection and amplification to finished product recovery. Cells and a culture solution are axially stirredin a tank body, so that radial shearing force is reduced as much as possible, the cells can be effectively protected and the cell yield is improved. According to the system, air is introduced separately, so that the content of each gas component in a culture process is stable, the condition that the cell culture is not directly influenced by external gas changes in a cell culture process can be ensured, and the content of each gas component is not changed; in the culture process, the culture temperature, the liquid amount and the gas concentration are controlled and the culture environment iskept stable; and meanwhile, manual operation is reduced, the cost is reduced, the risk of misoperation in the culture process is reduced and the culture efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a totally closed cell culture system. Background technique [0002] In recent years, CAR-T cell immunotherapy is considered to be one of the most promising treatments for cancer. It has many advantages that other therapies cannot match. For example, CAR-T cells can have multiple targeting sites, which can improve the accuracy of tumor treatment, and the action process is not limited by MHC (major histocompatibility complex); CAR-T cells kill The tumor range is wider, the effect is more durable; the technical attributes are strong, and the reproducibility is strong. In 2018, the FDA approved two CD19 CAR-T cell drugs (Kymriah and Yescarta, respectively), and these two CAR-T cell drugs have achieved good results in the treatment of malignant hematological tumors. However, CAR-T cell immunotherapy still has many limitations, such as the preparation of CAR-T cells. In the pro...

AI Technical Summary

Problems solved by technology

[0004] Most of the current cell cultures are manually operated. When a large number of cells need to be cultured for industrialization, a lot of labor and time costs are required. At the same time, as the burden on the operator increases, the risk of error is also greatly increased; in addition, Artificial culture cannot accurately control the environment of cell growth, which is not conducive to cell growth

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