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Urine stabilization protective agent and preparation method thereof

A technology for protecting reagents and urine, which is applied in the field of urine stable protection reagents and its preparation, which can solve the problems of complex urine components and easy changes, so as to overcome unfavorable factors, prevent degradation or oxidation, and inhibit enzyme degradation Effect

Inactive Publication Date: 2020-06-26
大连德泰克森生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the technical problem in the detection process of purine, uric acid and other metabolites in urine, because the urine composition is complex, the metabolites themselves are oxidized or degraded in the external environment after 2 hours, and the technical problem is very easy to change

Method used

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  • Urine stabilization protective agent and preparation method thereof
  • Urine stabilization protective agent and preparation method thereof

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Embodiment 1

[0029] Embodiment 1: For uric acid, purine, and the stable protective agent application and verification test of pH stability determination

[0030] The urine stabilizing and protecting reagent in this embodiment includes 0.67% of NaCl, 2% of methanol as a fixative, 0.02% of thymol, 0.6% of pyrogallic acid, 0.7% of kojic acid, and 0.08% of EDTA-2K. The urine stabilized protective reagent was adjusted to a pH measurement of 7.0 with sodium hydroxide.

[0031] The preparation method of above-mentioned urine stable preservation reagent is as follows:

[0032] In a container A, dissolve 0.67g NaCl in 90ml of distilled water to obtain a NaCl solution; dissolve 80mg of EDTA-2K solid in the above NaCl solution, then add 0.6g of pyrogallic acid and 0.7g of kojic acid in sequence, and mix . In container B was added 2 g of methanol and 20 mg of thymol. Pour the well-mixed mixture in container A into container B and mix, adjust the pH to 7.0 with sodium hydroxide, then pour it into a ...

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Abstract

The invention discloses a urine stabilization protective reagent. The urine stabilization protective reagent comprises NaCI, a fixing agent, a preservative, an antioxidant, kojic acid and / or flavone,EDTA dipotassium salt, sodium citrate and heparin. The urine stabilization protective reagent disclosed by the invention has the advantages that firstly, visible components in urine can be effectivelypreserved and are not easy to gather and crack, the degradation of enzymes is inhibited, and a guarantee is provided for cell or enzyme detection; secondly, the propagation and growth of microorganisms are controlled, premature degradation or oxidation of human metabolites after contact with the external environment is prevented, and conditions are provided for the accuracy of various detections;and thirdly, the protection of the natural acidity of the urine is noticed, and protective blending is carried out for urine acidity measurement. In conclusion, the invention provides a novel reliable method for stable preservation of a urine sample.

Description

technical field [0001] The present application relates to the field of urine specimen preservation, in particular to a urine stability protection reagent and a preparation method thereof. Background technique [0002] Urine is easy to obtain, can be collected noninvasively and can provide a wealth of information on the development of physiological changes. Urine is a fluid containing a large amount of metabolic end products produced by the kidneys, passed through the ureters, and discharged from the bladder. Its composition: water accounts for 96%-97%, the others are urea, uric acid, creatinine, ammonia and other non-protein nitrogen compounds, sulfate, etc. The urine is light yellow, with a specific gravity of 1.015-1.025. The pH of urine is affected by the nature of the food and changes greatly. Generally, it is pH 5.0-7.0, and the maximum range of fluctuation can reach pH 4.5-8.0. The urine output of normal people is between 1000 and 2000ml per day and night, and the d...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 王亚东张晓辉王若雨李梦阳姜海莲
Owner 大连德泰克森生物医药有限公司
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