An ielisa method for screening seronegative sheep of Mycoplasma ovine pneumonia and Mycoplasma capricolum subspecies cape pneumonia
A technology of mycoplasma pneumoniae and mycoplasma, which is applied in the direction of scientific instruments, instruments, measuring devices, etc., can solve the problems of decreased repeatability between batches, inapplicability to screening of a large number of samples, poor specificity and sensitivity of CFT, etc.
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[0017] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0018] 1. Main reagents
[0019] 96-well ELISA plate, Casein, BSA, skimmed milk powder, secondary antibody (rabbit anti-goat, sigma), McCP antibody detection cELISA kit (IDEXX company), microplate reader, BCA kit, etc. Strain: Mccp 1801 strain, preserved in Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Serum: collected at different time points in the vaccine / infection experiment, a total of 550 samples.
[0020] 2. Antigen preparation
[0021] Inoculate the 1801 strain of Mycoplasma goati pneumoniae (Mccp) 1801 preserved in the laboratory into the MTB medium, e...
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