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Method for producing coenzyme Q10 by combining plasma action with oxygen limitation

A plasma and coenzyme technology, applied in the field of microbial cultivation, can solve the problems of high power energy consumption and increased production costs, and achieve the effects of high mutation efficiency, simple and efficient operation, and reduced damage and lethal effects

Active Publication Date: 2020-06-16
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A high oxygen consumption rate can ensure the rapid growth of bacteria and the improvement of product synthesis rate, but the consumption of power energy in the production process is relatively large, which increases the production cost

Method used

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  • Method for producing coenzyme Q10 by combining plasma action with oxygen limitation
  • Method for producing coenzyme Q10 by combining plasma action with oxygen limitation
  • Method for producing coenzyme Q10 by combining plasma action with oxygen limitation

Examples

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Embodiment 1

[0065] This embodiment provides a method for producing coenzyme Q by combining the plasma action with oxygen limitation 10 The method comprises: the step of ARTP mutagenesis; Oxygen limitation screening coenzyme Q 10 Steps for High-Producing Strains; Fermentative Production of Coenzyme Q 10 steps; among them,

[0066] The step of described ARTP mutagenesis comprises:

[0067] The bacterial suspension of Rhodobacter sphaeroides is evenly covered on the surface of the slide, and then the slide is placed in the ARTP mutagenesis instrument, and the inert gas is used as the working gas for mutagenesis treatment and elution to obtain the mutagenized bacterial suspension;

[0068] The oxygen-limited screen for coenzyme Q 10 Steps for high-yielding strains, including:

[0069] Under sterile conditions, dilute the bacterium suspension after the mutagenesis, spread it on an oxygen-limited plate containing a plate medium supplemented with 0.2-0.4 g / L anhydrous sodium sulfite, and ca...

Embodiment 2

[0073] This embodiment provides a method for producing coenzyme Q by combining the plasma action with oxygen limitation 10 The method comprises: the step of ARTP mutagenesis; Oxygen limitation screening coenzyme Q 10 Steps for High-Producing Strains; Fermentative Production of Coenzyme Q 10 steps; among them,

[0074] The steps of the ARTP mutagenesis:

[0075] A suitable amount of rhodobacter sphaeroides bacterium suspension is evenly covered on the surface of the slide, and then the slide is placed in the ARTP mutagenesis instrument, with an inert gas as the working gas, at a carrier gas volume of 10SLM (SLM refers to liter per liter under standard conditions) Minutes) for 25s of mutagenesis, elution, the bacteria suspension after the mutagenesis can be obtained;

[0076] The oxygen-limited screen for coenzyme Q 10 Steps for high producing strains:

[0077] Under sterile conditions, the bacterial suspension after the mutagenesis was diluted and coated, grown on an oxyge...

Embodiment 3

[0082] This embodiment provides a method for producing coenzyme Q by combining the plasma action with oxygen limitation 10 The method comprises: the step of ARTP mutagenesis; Oxygen limitation screening coenzyme Q 10 Steps for High-Producing Strains; Fermentative Production of Coenzyme Q 10 steps; among them,

[0083] The step of described ARTP mutagenesis comprises:

[0084] The bacterial suspension of Rhodobacter sphaeroides is evenly covered on the surface of the slide, and then the slide is placed in the ARTP mutagenesis instrument, and the inert gas is used as the working gas for mutagenesis treatment and elution to obtain the mutagenized bacterial suspension;

[0085] The mutagenesis treatment specifically includes: mutagenesis for 25 seconds at a carrier gas volume of 10 SLM (SLM refers to liters per minute under standard conditions).

[0086] The mutated bacterial suspension was spread on a normal plate to grow, and a plate with a single colony was obtained.

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Abstract

The invention discloses a method for producing coenzyme Q10 by combining plasma action with oxygen limitation, which comprises the following steps: uniformly covering the surface of a slide with a bacterial suspension, putting the slide in an ARTP mutagenesis instrument, and carrying out mutagenesis treatment and elution to obtain the mutagenized bacterial suspension; diluting the mutated bacterial suspension under an aseptic condition, coating the diluted bacterial suspension on a flat plate containing a flat plate culture medium added with anhydrous sodium sulfite, and culturing to obtain aflat plate with a single colony; picking single colonies from the flat plate with the single colonies, putting the single colonies into a 24-pore plate, adding a seed culture medium, covering the 24-pore plate with a cover, and culturing to obtain a seed solution; and fermenting to produce the coenzyme Q10. The rhodobacter sphaeroides production strain with improved specific growth rate, oxygen affinity and product synthesis rate can be obtained and screened by combining plasma action with an oxygen limitation model, and the fermentation level of the coenzyme Q10 is effectively improved.

Description

technical field [0001] The invention relates to the technical field of microbial cultivation, in particular to a method for producing coenzyme Q by plasma action combined with oxygen limitation 10 Methods. Background technique [0002] coenzyme Q 10 (Coenzyme Q 10 ) is a fat-soluble compound widely present in the cell membrane of organisms, also known as ubiquinone (Ubiquinone), which acts as an electron carrier on the mitochondrial respiratory chain and is responsible for transferring electrons from complex I (or complex II) to Complex III. Studies have shown that coenzyme Q 10 It has physiological functions such as anti-oxidation, scavenging free radicals in the body, and promoting ATP synthesis. It is used as a drug for clinical treatment of cardiovascular diseases and a food additive. In the past two decades, its market demand has gradually increased. Coenzyme Q is produced by microbial methods 10 The development potential is huge. [0003] Rhodobacter sphaeroides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12N1/20C12P7/66C12R1/01
CPCC12N15/01C12N13/00C12N1/20C12P7/66
Inventor 庄英萍张幸子王泽建李丹陈必钦朱志春詹光煌杭海峰郭美锦
Owner EAST CHINA UNIV OF SCI & TECH
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