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Grease degradation complex microbial inoculant as well as preparation method and application thereof

A technology of compound bacterial agent and oil, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, etc. It can solve the problems of oil pollution and other problems, and achieve the effect of stable performance, reasonable formula and small investment

Inactive Publication Date: 2020-06-16
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to improve the treatment efficiency of grease degradation in view of the serious environmental pollution caused by grease pollutants

Method used

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  • Grease degradation complex microbial inoculant as well as preparation method and application thereof
  • Grease degradation complex microbial inoculant as well as preparation method and application thereof
  • Grease degradation complex microbial inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Grease degrading bacterial agent of the present invention is applied to the grease waste water of different concentration

[0046] Follow the steps below:

[0047] 1. Preparation of oil-degrading bacterial agent

[0048] Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, and Klebsiella mutans into beef extract peptone medium for seed preparation, culture for 2-3 days, and after the growth of the bacteria is stable, divide the bacteria according to Bacillus subtilis, Bacillus amyloliquefaciens bacillus and Klebsiella mutabilis seed solution (volume fraction, V / V) at a ratio of 5:2:3, further inoculated in QMM medium for seed expansion cultivation, so that the total number of effective viable bacteria was 2.5×10 9 CFU / mL, to obtain the oil-degrading compound bacterial agent.

[0049] 2. Simulated wastewater experiments with different oil concentrations

[0050] Using soybean oil as the main raw material, the oil-degrading composite bacterial agent wa...

Embodiment 2

[0051] Example 2 Grease Degradation Bacteria Applied to Grease Wastewater with Different pH

[0052] Follow the steps below:

[0053] 1. Preparation of oil-degrading compound bacterial agent:

[0054] 2. Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, Klebsiella mutans, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Lactobacillus casei and Saccharomyces cerevisiae into beef extract peptone culture Prepare the seeds in the culture medium and culture them for 2-3 days. After the growth of the bacteria is stable, the ratio of the seed liquid (V / V) of the above strains is 6:1:4:3:6:8:2:4, and then inoculate in QMM The medium is used for seed expansion cultivation, and a stable culture solution is obtained after two-level seed expansion cultivation, so that the total number of effective viable bacteria is 5×10 9 CFU / mL, to obtain the oil-degrading compound bacterial agent.

[0055] 3. Grease-degrading bacterial agents treat grease wastewater with different ...

Embodiment 3

[0057] Embodiment 3 Grease degrading bacterial agent is to the adaptation range of temperature

[0058] Follow the steps below:

[0059] 1. Preparation of oil-degrading compound bacterial agent:

[0060] Inoculate Bacillus subtilis, Bacillus amyloliquefaciens, Klebsiella mutans, Citrobacter, Pseudomonas stutzeri, Pseudomonas fluorescens, Lactobacillus casei and Saccharomyces cerevisiae into beef extract peptone medium Carry out seed preparation and culture for 2-3 days. After the growth of the strain is stable, the ratio of the seed solution (V / V) of the above strain is 1:4:3:1:6:8:2:1, and further inoculated in QMM medium Carry out seed expansion cultivation, obtain a stable culture medium after 2-level seed expansion cultivation, and adjust the OD of the bacterial suspension 600 is 0.8±0.1, and the oil-degrading composite bacterial agent is obtained.

[0061] 2. The removal rate of grease degrading bacteria agent at different temperatures:

[0062] Using soybean oil as t...

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Abstract

The invention belongs to the technical field of microbial degradation, and particularly relates to a grease degradation complex microbial inoculant as well as a preparation method and an application thereof. In order to reduce the pollution of grease pollutants to the environment, the invention provides the grease degradation complex microbial inoculant which comprises bacillus, Klebsiella and Citrobacter, and the effective viable count ratio of the bacillus to the Klebsiella to the Citrobacter is (2-8): (1-6): (1-4). The invention also provides the preparation method of the grease degradationcomplex microbial inoculant and the application of the grease degradation complex microbial inoculant in grease degradation. When in use, the strain is inoculated into oily wastewater or activated sludge according to an inoculation amount of 1.0-15% for degradation. The complex microbial inoculant is suitable for treating grease wastewater with various concentrations, and is also suitable for various sewage treatment technologies such as aerobic, facultative, anaerobic and the like. The complex microbial inoculant can be industrially applied on a large scale, and has the advantages of small investment, high efficiency, no secondary pollution and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation, and in particular relates to a compound bacterial agent for oil degradation and its preparation method and application. Background technique [0002] In recent years, people have paid more and more attention to the quality of the ecological environment, among which water pollution is the top priority of environmental pollution control. In 2017, my country's domestic sewage treatment capacity reached 46.26 billion tons, of which catering wastewater is an important part of domestic sewage. Although catering wastewater only accounts for about 3% of the total urban domestic sewage discharge, the BOD and COD it contributes account for 10%. More than 1 / 3 of the total pollutant content in wastewater. Due to the relatively small content of toxic substances in the waste water of the catering industry, it is often not paid attention to by people. Generally, it is directly discharged into the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/16C02F3/34C05F11/08C12R1/07C12R1/125C12R1/22C12R1/38C12R1/39C12R1/245C12R1/225C12R1/865C12R1/01C02F101/32
CPCC02F3/341C02F3/343C02F3/347C02F2101/32C05F11/08C12N1/16C12N1/18C12N1/20
Inventor 邱忠平邹美慧王强李星李明星陈文静
Owner SOUTHWEST JIAOTONG UNIV
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