A method for cultivating intelligent microbial streaking skills

Abstract

A method for cultivating intelligent microbial streaking skills, comprising the following steps: (1) Collecting the data in the table corresponding to the value of the culture medium and the streaking force, the specific operation is as follows: prepare medium with different concentrations, and use electronic inoculation by experienced operators The ring is drawn on different concentrations of culture medium, and the corresponding strength values ​​are recorded to form a database of the strength of the culture medium; The ring can display the force value when marking the line in real time, and the learner can compare it with the data in the database to judge whether the force of the line marking is within the standard range; (3) The intelligent petri dish can be displayed in real time according to the force of the electronic inoculation ring Outlined lines. This training method can shorten the time for learners to master the marking skills.

Description

technical field [0001] The invention relates to the technical field of cultivating microbial streaking skills, in particular to a method for cultivating intelligent microbial streaking skills. Background technique [0002] Existing teaching aids for cultivating microbial streaking skills include a glass petri dish and a metal inoculation loop used in conjunction with the glass petri dish. The glass petri dish is provided with a solid medium, and the front end of the metal inoculation loop is provided with a ring. Those who use a metal inoculation loop to draw a line on the solid medium of the glass culture dish, after marking, the glass culture dish needs to be cultivated in a constant temperature incubator for 24-120 hours, and the marking can only be observed after the colony grows; if not Ideally, the glass Petri dish after re-scribing still needs 24-120 hours of cultivation to observe the marking situation. The lag of the feedback time is inconvenient for beginners to co...

AI Technical Summary

Problems solved by technology

[0002] Existing teaching aids for cultivating microbial streaking skills include a glass petri dish and a metal inoculation loop used in conjunction with the glass petri dish. The glass petri dish is provided with a solid medium, and the front end of the metal inoculation loop is provided with a ring. Those who use a metal inoculation loop to draw a line on the solid medium of the glass culture dish, after marking, the glass culture dish needs to be cultivated in a constant temperature incubator for 24-120 hours, and the marking can only be observed after the colony grows; if not Ideally, the glass Petri dish after re-scribing still needs 24-120 hours of cultivation to observe the marking situation. The lag of feedback time is inconvenient for beginners to correct the deficiencies in time, and the teaching effect is often unsatisfactory

[0003] In the traditional experiment of cultivating microbial streaking skills, the glass petri dish needs to be sterilized by high pressure steam before use. After the experiment, from the perspective of biological safety, the used glass petri dish should also be sterilized by high pressure steam before the inside can be sterilized. The culture medium is poured out for scrubbing; high-pressure steam sterilization and constant temperature culture both consume electric energy, and the detergent and water consumed by washing the petri dish are discharged into the environment, and the dumping of the culture medium pollutes the environment; Repeat the marking 5 times (that is, use 5 petri dishes, but some beginners even need more than 10 petri dishes) to master the marking skills to measure. The annual energy consumption and pollutants entering the environment due to this work are Noteworthy Values

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