Intelligent microbial streak skill culture method

Abstract

The invention discloses an intelligent microorganism streak skill culture method. The method comprises the following steps: (1) data of a culture medium and streaking force numerical value correspondence table is collected, specifically, culture media with different concentrations are prepared, the culture media with different concentrations are streaked by an experienced operator through an electronic inoculation ring, and corresponding force numerical values are recorded to form a culture medium streaking force database; (2) a learner uses an electronic inoculating loop to streak the intelligent culture dish, the electronic inoculating loop can display the intensity value during line drawing in real time, and the learner compares the intensity value with the data of the database to judgewhether the line drawing intensity of the learner is within a standard range or not; and (3) the streak shape can be displayed on the intelligent culture dish in real time according to the strength of the electronic inoculating loop. The culture method can shorten the time for learners to master the scribing skills.

Description

technical field [0001] The invention relates to the technical field of cultivating microbial streaking skills, in particular to a method for cultivating intelligent microbial streaking skills. Background technique [0002] Existing teaching aids for cultivating microbial streaking skills include a glass petri dish and a metal inoculation loop used in conjunction with the glass petri dish. The glass petri dish is provided with a solid medium, and the front end of the metal inoculation loop is provided with a ring. Those who use a metal inoculation loop to draw a line on the solid medium of the glass culture dish, after marking, the glass culture dish needs to be cultivated in a constant temperature incubator for 24-120 hours, and the marking can only be observed after the colony grows; if not Ideally, the glass Petri dish after re-scribing still needs 24-120 hours of cultivation to observe the marking situation. The lag of the feedback time is inconvenient for beginners to co...

AI Technical Summary

Problems solved by technology

[0002] Existing teaching aids for cultivating microbial streaking skills include a glass petri dish and a metal inoculation loop used in conjunction with the glass petri dish. The glass petri dish is provided with a solid medium, and the front end of the metal inoculation loop is provided with a ring. Those who use a metal inoculation loop to draw a line on the solid medium of the glass culture dish, after marking, the glass culture dish needs to be cultivated in a constant temperature incubator for 24-120 hours, and the marking can only be observed after the colony grows; if not Ideally, the glass Petri dish after re-scribing still needs 24-120 hours of cultivation to observe the marking situation. The lag of feedback time is inconvenient for beginners to correct the deficiencies in time, and the teaching effect is often unsatisfactory

[0003] In the traditional experiment of cultivating microbial streaking skills, the glass petri dish needs to be sterilized by high pressure steam before use. After the experiment, from the perspective of biological safety, the used glass petri dish should also be sterilized by high pressure steam before the inside can be sterilized. The culture medium is poured out for scrubbing; high-pressure steam sterilization and constant temperature culture both consume electric energy, and the detergent and water consumed by washing the petri dish are discharged into the environment, and the dumping of the culture medium pollutes the environment; Repeat the marking 5 times (that is, use 5 petri dishes, but some beginners even need more than 10 petri dishes) to master the marking skills to measure. The annual energy consumption and pollutants entering the environment due to this work are Noteworthy Values

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