3D voxel printing device based on cell soft spheres and method thereof

Abstract

The invention discloses a 3D voxel printing device based on cell soft spheres and a method thereof. The device comprises a water phase input part, an oil phase input part and a microfluidic microtube,wherein the water phase input part inputs an extracellular matrix material loaded with cells, namely bio-ink, into the microfluidic microtube; and the oil phase input part synchronously inputs a low-viscosity and low-boiling-point oil phase meeting set requirements into the microfluidic microtube. The extracellular matrix material loaded with the cells is separated into monodisperse microdropletsembedded with the cells through an oil phase, the microdroplets are gelled into cell soft spheres in the flowing process in the microtube, the cell soft spheres are sequentially shot onto a substrateby utilizing rapid expansion and volatilization of the oil phase at an outlet of the microfluidic microtube, and the cell soft spheres are adhered together through an incompletely gelled ink material. An in-vitro model capable of highly reducing the human organ or tissue structure and the cell microenvironment can be printed under the condition that a printing material does not contain a biological inert material for assistance.

Description

technical field [0001] The invention relates to 3D bioprinting, in particular to a 3D voxel printing device and method based on soft cell spheres. Background technique [0002] Bio-ink cell soft scaffold materials are derived from mammalian tissue extraction materials, rich in protein fibers, which can provide human cells with an in vivo-like matrix environment, and are an ideal matrix material for tissue preparation in vitro. However, in 3D bioprinting based on protein fibers, it is difficult to independently shape the printed structure, and it is difficult to achieve 3D printing of biological soft tissues without the aid of biologically inert materials that only contain cells and corresponding extracellular matrix materials. Contents of the invention [0003] The main purpose of the present invention is to overcome the above-mentioned technical defects and provide a 3D voxel printing device and method based on soft cell spheres. [0004] To achieve the above object, the...

AI Technical Summary

Problems solved by technology

However, in the 3D bioprinting based on protein fibers, it is difficult to independently shape the printed structure, and it is difficult to achieve 3D printing of biological soft tissues without the aid of biologically inert materials that only contain cells and corresponding extracellular matrix materials.

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