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Banana transcription factors maarf12, maarf24 and their application in inhibiting the expression of masbe2.3

A transcription factor, banana technology, applied in the direction of DNA / RNA fragments, application, transferase, etc., to achieve the effect of improving starch quality and regulating amylopectin content

Active Publication Date: 2021-08-03
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether ARF can interact with SBE gene and regulate its expression has not been reported yet.

Method used

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  • Banana transcription factors maarf12, maarf24 and their application in inhibiting the expression of masbe2.3
  • Banana transcription factors maarf12, maarf24 and their application in inhibiting the expression of masbe2.3
  • Banana transcription factors maarf12, maarf24 and their application in inhibiting the expression of masbe2.3

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Embodiment Construction

[0027] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] 1. Gene acquisition

[0029] 1. Acquisition of banana transcription factor MaARF12 gene

[0030] Using the cDNA of banana fruit as a template, the

[0031]5'-ATGAAGCTTTCCACTGTTGGTATC-3'

[0032] 5'-CTAACAGAAAGAGTCAACTCCCT-3'

[0033] As a primer, one obtained by PCR amplification has a base sequence of 2,406 bp, and its sequence is shown in SEQ ID No.1.

[0034] 2. Acquisition of banana transcription factor MaARF24 gene

[0035] Using the cDNA of banana fruit as a template, the

[0036] 5'-ATGGCGTCGTCGGAGGTGTCGGT-3'

[0037] 5'-TCAAAAGGCCGCATCAACTCCAG-3'

[0038] As a primer, one obtained by PCR amplification has a base sequence of 2,...

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Abstract

The present invention provides banana transcription factors MaARF12 and MaARF24, which are proteins encoded by genes whose nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The present invention also provides the encoding gene and application of the above transcription factor. Both the banana transcription factors MaARF12 and MaARF24 of the present invention can be combined with the promoter region of the banana fruit starch branching enzyme gene MaSBE2.3, can interact with the MaSBE2.3 gene, and inhibit its expression, which is beneficial to regulating the content of amylopectin and improving starch It is of great significance to cultivate new banana varieties with high quality and provide candidate transcription factor resources for improving the quality of amylopectin in bananas or other fruits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to banana transcription factors MaARF12, MaARF24 and their application in inhibiting the expression of MaSBE2.3. Background technique [0002] Plant starch is the main source of human nutrition and energy. It consists of two parts, amylose and amylopectin. Amylopectin accounts for about 70-80% of the total starch content and is a key factor affecting starch quality and processing performance (silver Yongan et al., 2018). Amylopectin biosynthesis is mainly determined by three enzymes: soluble starch synthase (SS), starch branching enzyme (SBE) and starch debranching enzyme (DBE) (Myers et al., 2000). SBE mainly controls the branching pattern of amylopectin, and ultimately affects starch quality, yield and nutritional value (Kim et al., 1999; Tetlow et al., 2014). At the same time, SBE gene expression is involved in multiple biological processes such as plant growth, develop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N15/113C12N15/11A01H5/08A01H6/00
CPCC07K14/415C12N9/107C12N15/8218C12N15/8235C12N15/8245C12Y204/01018
Inventor 苗红霞孙佩光金志强徐碧玉刘菊华贾彩红赵东方
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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