Method for detecting exogenous beet sugar in honey

Abstract

The invention provides a method for detecting exogenous beet sugar in honey, and belongs to the field of food detection. The method comprises the following steps: 1) selecting a raw honey sample, removing moisture, preparing an aqueous solution of the raw honey sample, fermenting, converting the raw honey sample into ethanol, measuring the hydrogen isotope ratio of the ethanol, and constructing ahydrogen isotope ratio database of the ethanol converted from the raw honey; 2) removing water from the to-be-detected honey, preparing an aqueous solution of the to-be-detected honey, fermenting, converting the to-be-detected honey into ethanol, and determining the hydrogen isotope ratio of the ethanol; and 3) comparing the hydrogen isotope ratio measurement result of the ethanol of the honey tobe measured in the step 2) with the hydrogen isotope ratio database of the ethanol of the raw honey sample obtained in the step 1), and if the measurement result of the honey to be measured is smallerthan the minimum value of the database, determining that beet sugar is added into the honey to be measured. The method is mainly used for detecting the exogenous beet sugar in the honey and effectively solves the technical problem of lack of a simple, rapid and low-cost beet sugar detection method in the field of honey.

Description

technical field [0001] The invention belongs to the field of food detection and relates to a method for detecting exogenous beet sugar in honey. Background technique [0002] Honey is a popular health food. After decades of development, my country's honey industry has made great achievements: at present, the total number of bee colonies in my country has reached 9.2 million, accounting for 13% of the total number of bee colonies in the world. my country's honey production rose from 411,800 tons in 2011 to 542,500 tons in 2018, ranking first in the world, and its export volume was 129,300 tons, a year-on-year increase of 0.74%, accounting for 1 / 4 of the world's honey trade volume. [0003] At present, my country's bee industry has entered the track of rapid development. More than 1,300 bee product processing enterprises of different sizes have obtained national certification, including seven national-level leading enterprises, 35 provincial-level leading enterprises, and indu...

AI Technical Summary

Problems solved by technology

[0007]However, there are two major disadvantages in this technology: ①The above-mentioned micro-distillation system needs to be specially customized to achieve a recovery rate higher than 96.5%, and ethanol concentration in the fraction higher than 95%v / V requirements, the micro-distillation system is a patented product of the French company Eurofins, a single set costs 800,000 RMB, and requires a high level of proficiency in the operator during use. Even so, the time for a single distillation also needs more than 4 hours; ② The nuclear magnetic resonance instrument measures the hydrogen isotope ratio of ethyl alcohol methyl, and its principle is to determine the absolute content of deuterium at the methyl site in the quantitative sample. Since the content of deuterium in the natural state only accounts for 0.02% of hydrogen atoms, in order to obtain sufficient information Noise ratio and stability, the determination time of a single sample is as long as 5h or more

The above two defects lead to low analysis efficiency of this technology, and there is no unit in our country that can use this technology, which seriously hinders the research and application in the field of honey adulteration detection in our country