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Preparation method and application of novel phenolic acid compound with anti-inflammatory activity

A technology of compounds and phenolic acids, which is applied in the field of preparation of anti-inflammatory drugs, can solve problems such as long application time and tolerance

Active Publication Date: 2020-06-09
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these two types of anti-inflammatory drugs have good clinical application effects, long-term large-scale use will cause a series of adverse reactions or side effects, such as gastric mucosal damage, liver damage, kidney damage, etc., and long-term use can also lead to tolerance sex

Method used

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  • Preparation method and application of novel phenolic acid compound with anti-inflammatory activity
  • Preparation method and application of novel phenolic acid compound with anti-inflammatory activity
  • Preparation method and application of novel phenolic acid compound with anti-inflammatory activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing this phenolic acid compound comprises the steps:

[0026] (1) Heat and reflux the stems and leaves of Xiaohuaqingfengteng with 12 times the amount of 70% ethanol solution to extract 3 times, each time for 2 hours, filter, and concentrate the filtrate under reduced pressure to obtain a crude extract;

[0027] (2) Dissolve the crude extract obtained in step (1) with 20% ethanol, and filter to obtain the supernatant;

[0028] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 70 % ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. E was subjected to preparativ...

Embodiment 2

[0030] The method for preparing the compound of formula 1 structure, comprises the steps:

[0031] (1) Heat and reflux the stems and leaves of Xiaohuaqingfengteng with 15 times the amount of 95% ethanol solution to extract 3 times, each time for 2 hours, filter, and concentrate the filtrate under reduced pressure to obtain a crude extract;

[0032] (2) Dissolve the crude extract obtained in step (1) with 20% ethanol, and filter to obtain the supernatant;

[0033] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 70 % ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. E was subjected to p...

Embodiment 3

[0035] The method for preparing the compound of formula 1 structure, comprises the steps:

[0036] (1) The stems and leaves of Xiaohuaqingfengteng were extracted twice by cold soaking with 30 times the amount of 70% ethanol solution, each time for 24 hours, filtered, and the filtrate was concentrated under reduced pressure to obtain a crude extract;

[0037] (2) Dissolve the crude extract obtained in step (1) with 20% ethanol, and filter to obtain the supernatant;

[0038] (3) Take the supernatant obtained in step (2) and load it on HP-20 macroporous resin column (sample:resin is filled according to the volume ratio of 1:3), followed by water, 30% ethanol, 50% ethanol, 70 % ethanol, ethanol elution, 3 column volumes for each gradient elution. Take the eluted part of 30% ethanol and pass it through ODS medium and low pressure reversed phase liquid chromatography column (50*500mm), MeOH-H 2 O (20%, 30%, 40%, 50%, 60%, 70%, 80%) was eluted to obtain 7 fractions A-G. E was subj...

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Abstract

The invention relates to application of a novel phenolic acid compound in preparation of an anti-inflammatory drug, and also relates to a preparation method for extracting, separating and purifying the compound from a traditional Chinese medicine sabia parviflora. Pharmacological research shows that the novel compound can remarkably inhibit the levels of NO, IL-1, IL-6 and TNF-alpha in mouse macrophages RAW246.7 caused by lipopolysaccharide (LPS), and the inhibition effect is mostly dependent on a dose, so the compound has obvious anti-inflammatory activity and can be used for preparing anti-inflammatory drugs. The preparation method is simple and easy to implement, and the obtained compound is high in purity and has good economic benefits and application prospects.

Description

technical field [0001] The technical field of traditional Chinese medicine of the present invention specifically relates to the preparation method of extracting and separating phenolic acid compounds from the stems and leaves of Qingfeng vine and the preparation method of Chinese medicine extracts or mixtures containing the compounds and the application in the preparation of anti-inflammatory drugs. Background technique [0002] Xiaohua Qingfengteng is a liana of Qingfeng rattan family Qingfeng rattan, Xiaohua Qingfengteng Sabia parviflora Dried stems and leaves of Wall.ex Roxb. The small flower Qingfengteng produced in Guizhou is a folk medicine of the Buyi and Miao nationalities. It is mainly distributed in Xingyi City, Anlong County, Ceheng County, Wangmo County and other places in Guizhou. Its rhizomes and leaves can be used as medicine. Anti-inflammatory and analgesic effect. [0003] Macrophages are white blood cells in tissues, derived from monocytes. Both macropha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C65/19C07C51/42A61P29/00
CPCA61P29/00C07C51/42C07C65/19
Inventor 李志峰冯育林王琦
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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