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Preparation method for Taisui fermentation liquid, Taisui fermentation liquid and application of Taisui fermentation liquid

A fermented liquid and Tai Sui technology, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as limitations of functional ingredients, slow growth of Tai Sui meat, etc.

Inactive Publication Date: 2020-06-09
白山太岁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow growth of Tai Sui meat, the separation and extraction of functional components from Tai Sui meat is limited

Method used

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  • Preparation method for Taisui fermentation liquid, Taisui fermentation liquid and application of Taisui fermentation liquid
  • Preparation method for Taisui fermentation liquid, Taisui fermentation liquid and application of Taisui fermentation liquid
  • Preparation method for Taisui fermentation liquid, Taisui fermentation liquid and application of Taisui fermentation liquid

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preparation example Construction

[0025] The invention provides a preparation method of Tai Sui fermented liquid, comprising the following steps:

[0026] 1) mixing kiwifruit, plums, strawberries and Ganoderma lucidum, beating, and filtering to obtain a filtrate, and mixing the filtrate with honey to obtain Tai Sui culture fluid;

[0027] 2) Ferment the Tai Sui culture fluid obtained in the step 1) and Tai Sui after mixing to obtain a fermented product, remove the Tai Sui and filter to obtain the Tai Sui fermentation liquid;

[0028] The mass ratio of the kiwi fruit, plum, strawberry, ganoderma lucidum and honey is 5-10:5-10:5-10:1-2:0.5-1.

[0029] The conditions of mixing, beating and filtering are not particularly limited in the invention, and conventional methods can be used. In the present invention, the function of the kiwi fruit is to provide carbohydrates, vitamins and trace elements, especially vitamin C and vitamin A for the growth of Tai Sui; the function of the plum is to provide sugar, protein, f...

Embodiment 1

[0037] Mix 500 grams of kiwi fruit, 500 grams of plums, 500 grams of strawberries and 100 grams of Ganoderma lucidum powder (150 mesh), beat and filter to obtain a filtrate, and fully stir the filtrate and 50 grams of honey to obtain a nutrient solution. Mix wild Taisui: nutrient solution: mineral water in a weight ratio of 1:100:100, cultivate for 1 month, cultivate at a temperature of 25°C, and have a pH value of 2.5. After the cultivation, use a membrane filtration method (0.45 micron) to filter and separate. The filtrate is the Tai Sui fermentation broth. Conventional analysis methods were used to detect pyrroloquinoline quinone, protein, crude polysaccharide and nucleic acid in Taisui fermentation broth. The results are shown in Table 1.

[0038] Table 1 The detection results of Taisui fermentation broth

[0039]

[0040]

Embodiment 2

[0042]Effects of different concentrations of Ganoderma lucidum fermentation broth on the lifespan of wild-type Caenorhabditis elegans, Caenorhabditis elegans culture: the Caenorhabditis elegans used are all wild-type Caenorhabditis elegans (TheBristolN2 strain), with E.coli OP50 as food, in NGM (Nematode Growth Medium) culture medium, cultured in a constant temperature incubator at 20°C. In order to ensure that the physiological state of C. elegans is similar in the same batch of experiments, it is necessary to synchronize the C. elegans before the experiment. Rinse the C. Precipitate, remove the supernatant, add M9 buffer again to the precipitate, and wash it repeatedly once. After the washing is completed, remove the excess M9 buffer, keep the Caenorhabditis elegans precipitate, and add 1mL lysate to it, lyse for 3 minutes, and use Hand centrifuge, centrifuge at low speed, remove the lysate, wash M9 once, remove excess liquid, use a pipette to draw the precipitate into the N...

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Abstract

The invention provides a preparation method for a Taisui fermentation liquid, the Taisui fermentation liquid and an application of the Taisui fermentation liquid, and belongs to the technical field ofmicrobial fermentation. The preparation method includes the following steps: 1) mixing kiwi fruits, plums, strawberries and glossy ganoderma, performing beating, performing filtration to obtain a filtrate, and mixing the filtrate and honey to obtain a Taisui culture solution; and 2) mixing the Taisui culture solution obtained in the step 1) and Taisui, performing fermentation to obtain a fermented product, removing Taisui, and performing filtration to obtain the Taisui fermentation liquid, wherein the mass ratio of the kiwi fruits to the plums to the strawberries to the glossy ganoderma to the honey is (5-10):(5-10):(5-10):(1-2):(0.5-1). According to the Taisui fermentation liquid prepared by the preparation method provided by the invention, the content of the functional components in theTaisui fermentation liquid is improved, and the Taisui fermentation liquid has a life-prolonging effect on caenorhabditis elegans, and has a wrinkle-removing function.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method of Tai Sui fermentation liquid, Tai Sui fermentation liquid and its application. Background technique [0002] Taisui is also known as Ganoderma lucidum. Li Shizhen's "Compendium of Materia Medica" recorded Ganoderma lucidum, and included it in the "Ganoderma" category of the "Dish" department. It can be eaten and used as medicine. Not old, life-prolonging immortal". According to the "Shen Nong's Materia Medica" records: "Ganoderma lucidum is non-toxic, nourishes the middle, nourishes essence, increases wisdom, cures knots in the chest, and makes light of body and youth after long-term use." "Shan Hai Jing" called it "Shirou", "Jurou", "Tai Sui", and "Feng". They were the health-preserving delicacies of ancient emperors. Tai Sui is very rare and is the top grade of all medicines. According to ancient records, Tai Sui is mild in n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P39/06A61P17/00A61K8/9728A61Q19/08A61Q19/00
CPCA61K8/9728A61K36/07A61K2236/19A61K2800/85A61P17/00A61P39/06A61Q19/00A61Q19/08
Inventor 岳俊峰李家才宋旭昆
Owner 白山太岁生物科技有限公司
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