Method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials

A technology for Tenebrio militaris and Cordyceps militaris is applied in the field of cultivating and obtaining fruiting bodies of Cordyceps militaris, can solve problems such as unsatisfactory biological conversion efficiency, unsatisfactory, unfavorable industrialized production, etc., and achieves the benefits of industrialized production development and improved medicinal components. Effect

Inactive Publication Date: 2020-06-05
大连市现代农业生产发展服务中心
View PDF11 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, CN100392063C discloses the Cordyceps militaris sporocarp with Tenebrio molitor pupae as the host and its cultivation method, the Tenebrio molitor pupae is the host, injection inoculation is inconvenient to operate and is not conducive to factory production, and there are few sporocarps; CN103688760A discloses a A method for cultivating artificial Cordyceps using Tenebrio molitor as a carrier. It is inconvenient to operate by injection and inoculation, mainly to harvest infected insects, and the small amount of inoculation causes no fruiting body or is extremely short; CN103749149A discloses a method for using Tenebrio molitor pupae as The method for cultivating artificial Cordyceps with a carrier, which uses pupae as the host, and inoculates by injection only after surface disinfection with alcohol, there is a risk of carrying pathogenic bacteria in the pupae, and the fruiting bodies are short; A method for cultivating Cordyceps products with a carrier, which uses Tenebrio molitor pupae as a host, injects and inoculates, and obtains a product that is a Cordyceps worm without fruiting bodies; CN108184540A discloses a method for cultivating Cordyceps militaris with live Tenebrio molitor larvae as a host, and the obtained fruiting bodies 1.8-3cm, and it is inoculated by injection
[0004] In the above-mentioned related prior art, injection infection is often used to simply obtain the mycelia of Cordyceps militaris. On the one hand, there are cumbersome operations, unsatisfactory biological conversion efficiency, and lack or poor nutritional components of the obtained entity, such as no There are very few fruiting bodies or fruiting bodies and the fruiting bodies are only 1.8~3cm. On the other hand, the existing processing methods are not conducive to industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials
  • Method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials
  • Method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Acquisition of the mother species of Cordyceps militaris: Mature wild individuals of Cordyceps militaris that have not yet scattered spores were collected from the Changbai Mountains in Northeast my country, and were separated from tissues in the laboratory to obtain high-quality strains of the mother species of Cordyceps militaris.

[0035] 2. Liquid medium preparation: The medium formula is peeled potato 18%, glucose 2%, KH 2 PO 4 0.05%, MgSO 4 ·7H 2 O 0.05%, peptone 0.4%, and the rest is water. During the preparation process, it is necessary to supplement the water lost by evaporation, and the amount of supplemented water matches the total amount of the liquid medium.

[0036] Weigh the peeled potatoes and cut them into 2mm slices, boil them in boiling water at 120°C for 15 minutes, until they are crispy but not rotten, filter them with 6 layers of gauze, add glucose and KH to the filtrate 2 PO 4 , MgSO 4 ·7H 2 O, peptone, water, divided into small infusio...

Embodiment 2

[0051] 1. Acquisition of the mother species of Cordyceps militaris: Mature wild individuals of Cordyceps militaris that have not yet scattered spores were collected from the Changbai Mountains in Northeast my country, and were separated from tissues in the laboratory to obtain high-quality strains of the mother species of Cordyceps militaris.

[0052] 2. Liquid medium preparation: The medium formula is 25% peeled potato, 4% glucose, KH 2 PO 4 0.2%, MgSO 4 ·7H 2 O 0.2%, peptone 0.5%, the rest is water. During the preparation process, it is necessary to supplement the water lost by evaporation, and the amount of supplemented water matches the total amount of the liquid medium.

[0053] Weigh the peeled potatoes and cut them into 3mm slices, boil them in boiling water at 100°C for 18 minutes, until they are crisp but not rotten, filter them with 8 layers of gauze, and add glucose, KH 2 PO 4 , MgSO 4 ·7H 2 O, peptone, water, divided into small infusion bottles with a capac...

Embodiment 3

[0068] 1. Acquisition of the mother species of Cordyceps militaris: Mature wild individuals of Cordyceps militaris that have not yet scattered spores were collected from the Changbai Mountains in Northeast my country, and were separated from tissues in the laboratory to obtain high-quality strains of the mother species of Cordyceps militaris.

[0069] 2. Liquid medium preparation: The medium formula is 20% peeled potato, 1% glucose, KH 2 PO 4 0.15%, MgSO 4 ·7H 2 O 0.15%, peptone 0.3%, the rest is water. During the preparation process, it is necessary to supplement the water lost by evaporation, and the amount of supplemented water matches the total amount of the liquid medium.

[0070] Weigh the peeled potatoes and cut them into 3mm slices, boil them in boiling water at 110°C for 20 minutes, and when they are crispy but not rotten, filter them with 8 layers of gauze, and add glucose and KH to the filtrate 2 PO 4 , MgSO 4 ·7H 2 O, peptone, water, divided into small infus...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials. The method comprises the steps of inoculating a liquid nutrient medium with cordyceps militaris parent strains, and performing culturing with a total air temperature vibrating shaking table to obtain a liquid strain, wherein the formula of the liquid nutrient medium comprises 18%-25% of potatoes, 1%-4% of glucose, 0.05%-0.2% of KH2PO4, 0.05%-0.2% of MgSO4.7H2O, 0.2%-0.5% of peptone and the balance water; adding yellow mealworm larvae to a culture flask,adding a glucose solution of which the pH is 8 and the concentration is 1%, performing sealing, and performing high-temperature high-pressure sterilization to obtain the culture medium for culturingthe yellow mealworms; uniformly spraying the liquid strain to the surface of the culture medium for culturing the yellow mealworms; and placing the culture flask on a culturing chamber frame, performing culturing, performing picking when ascocarp is densely distributed at the middle and upper part of the sporophore of the cordyceps militaris and spores are not dispersed, and separating yellow mealworm cakes from the cordyceps militaris sporophore, so as to obtain the cordyceps militaris sporophore. The technology is simple, industrial operation is easy to realize, the yellow mealworm larvae are used as main materials for culturing, the obtained cordyceps militaris sporophore is rich in ascocarp and ascospore, and the content of nutrient components of cordycepin, cordyceps sinensis polysaccharide, SOD enzymes and the like is high.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a method for cultivating and obtaining fruiting bodies of Cordyceps militaris by using Tenebrio molitor larvae as raw materials. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps genus Ergotaceae of the Ascomycetes class Hypocreaes. Because the main active ingredient is similar to or higher than that of Cordyceps sinensis, and has relatively mature cultivation conditions, it has become an effective substitute for Cordyceps sinensis. [0003] In the prior art, CN100392063C discloses the Cordyceps militaris sporocarp with Tenebrio molitor pupae as the host and its cultivation method, the Tenebrio molitor pupae is the host, injection inoculation is inconvenient to operate and is not conducive to factory production, and there are few sporocarps; CN103688760A discloses a A method for cultivating artificial Cor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 常广宁赵彩云侯秀明许文博常晓娇吉志新温晓蕾孔繁辉陈雪
Owner 大连市现代农业生产发展服务中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap