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Hydrogel for repairing peripheral nerve injury and preparation method of hydrogel

A peripheral nerve injury and hydrogel technology, applied in the field of biomedical engineering materials, can solve problems such as the effect of cross-linking agent PNI repair, and achieve the effect of prolonging the treatment period, achieving targeting, and definite curative effect.

Inactive Publication Date: 2020-06-02
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that GEL and HA gel materials were prepared by using EDC / NHS as a cross-linking agent, however, the toxicity of the cross-linking agent may have an adverse effect on PNI repair

Method used

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  • Hydrogel for repairing peripheral nerve injury and preparation method of hydrogel
  • Hydrogel for repairing peripheral nerve injury and preparation method of hydrogel
  • Hydrogel for repairing peripheral nerve injury and preparation method of hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A kind of PLGA drug-loaded microspheres, the preparation method is as follows:

[0063] (1) Dissolve 0.3g of PVA in 300mL of deionized water, and dissolve it completely at 70°C to form a PVA solution;

[0064] (2) Dissolve 0.3g PLGA in 3.5mL dichloromethane solution to form PLGA solution;

[0065] (3) Dissolve NGF in PBS buffer solution to make 200ng / mL NGF solution, take 500 μL of NGF solution and drop into 3mL of PLGA solution to obtain PLGA / NGF mixed solution;

[0066] (4) Cool down the PVA solution and the PLGA / NGF mixed solution in an ice bath, take 500 μL of PBS buffer solution and add it to the PLGA / NGF mixed solution, and use an ultrasonic cell disruptor to ultrasonically disperse for 20 seconds at a power of 200 W until the liquid is milky white;

[0067] (5) Drop the above solution into 24mL PVA solution with a dropper, continue to ultrasonically disperse for 2min until the liquid is pale milky white, then place it on a magnetic stirrer and stir for 5h to com...

Embodiment 2

[0072] A CS-HEC-HA / GP hydrogel, the preparation method of which is:

[0073] (1) Stir and dissolve 40mg HA into 8mL distilled water to form HA solution;

[0074] (2) Add 20 mg of HEC into the HA solution, and continue to stir until the HEC is completely dissolved to form a mixed solution;

[0075] (3) Add 200mg CS into the mixed solution, stir evenly, add 1mL of 1mol / L hydrochloric acid solution, continue to stir until the CS is completely dissolved to form a CS / HEC / HA mixed solution, and store it at 4°C for later use;

[0076] (4) Slowly add 1 mL of 30% GP solution to 8 mL of CS / HEC / HA mixed solution dropwise, and continue to stir in the ice bath for 10 minutes to mix evenly;

[0077] (5) The above solution was poured into the mold, frozen in a refrigerator at -20°C, and dried in vacuum to obtain the CS-HEC-HA / GP hydrogel.

[0078] The schematic diagrams of gelation of the CS-HEC-HA / GP hydrogel prepared in this example at 25°C and 37°C are as follows image 3 shown. It ca...

Embodiment 3

[0081] A CS-HEC-HA(20) / GP(PLGA@NGF) hydrogel, comprising the following components in mass percent: 0.2% hyaluronic acid, 0.2% sodium hydroxyethylcellulose, 2% chitosan, 3% sodium β-glycerophosphate, 1% PLGA drug-loaded microspheres, and the balance is water. The preparation method of the PLGA drug-loaded microspheres is the same as in Example 1.

[0082] Its preparation method is:

[0083] (1) Stir and dissolve 20mg HA into 8mL distilled water to form HA solution;

[0084] (2) Add 20 mg of HEC into the HA solution, and continue to stir until the HEC is completely dissolved to form a mixed solution;

[0085] (3) Add 200mg CS into the mixed solution, stir evenly, add 1mL of 1mol / L hydrochloric acid solution, continue to stir until the CS is completely dissolved to form a CS / HEC / HA mixed solution, and store it at 4°C for later use;

[0086](4) Slowly add 1mL of 30% GP solution into 8mL CS / HEC / HA mixed solution dropwise. After the addition is completed, continue to stir in an i...

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Abstract

The invention relates to a hydrogel for repairing peripheral nerve injury (PNI). The hydrogel contains the following components in percentages by mass: 0.2%-0.6% of hyaluronic acid, 0.18%-0.22% of hydroxyethyl cellulose sodium, 1.5%-2.5% of chitosan, 2.5%-3.5% of beta-glycerophosphoric acid disodium salt, 0.8%-1.2% of PLGA drug-carrying microspheres, and the balance of water. The hydrogel providedby the invention uses CS-HEC-HA / GP as a substrate, overcomes the shortcomings of a single component HA scaffold, improves the mechanical properties of the substrate, controls the gelation time and degradation, belongs to temperature-sensitive hydrogels, and can be used for injection administration; and in addition, a nerve growth factor (NGF) is loaded into a biological material, so that targetedand sustained molecular release can be realized directly in tissue repair, and the NGF is loaded into the PLGA microspheres to obtain PLGA (NGF) with a uniform particle size to achieve the effect ofdrug sustained release and prolong the treatment cycle by the hydrogel, so that side effects caused by drug burst release can be avoided.

Description

technical field [0001] The invention relates to a hydrogel for repairing peripheral nerve damage and a preparation method thereof, belonging to the field of biomedical engineering materials. Background technique [0002] The repair effect of peripheral nerve injury (PNI) has always been a difficult problem for orthopedic surgeons. End-to-end suturing can be used to treat short-distance nerve defects. The "gold standard" for the treatment of long-segment nerve defects is autologous nerve transplantation. Defects such as nerve mismatch limit its clinical application. Tissue-engineered nerve guides have been approved for clinical use, and some progress has been made in the treatment of PNI. However, in the case of long-segment nerve defects, the empty nerve conduit lacks fillers such as extracellular matrix and nerve growth factor to improve the microenvironment in the lumen of the nerve conduit, and the therapeutic effect is not good. At present, a variety of biological mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/52A61L27/54A61L27/22A61L27/50A61L27/20A61L27/18
CPCA61L27/12A61L27/18A61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L2300/414A61L2300/602A61L2400/06A61L2430/32C08L89/00C08L5/08C08L1/284C08L67/04
Inventor 冯龙宝王梦颖蓝咏刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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