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Non-enzymatic glucose sensing detection system

A detection system, glucose technology, applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve problems such as chemical instability, operational complexity, poor reproducibility, loss of characteristics, etc.

Active Publication Date: 2020-05-29
融创达科技成果转化(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Enzymatic glucose biosensors using glucose enzymes such as glucose oxidase have found that their chemical instability, operational complexity, and poor reproducibility are the main obstacles to their widespread application, and they cannot be used below pH2 and above pH8. Use, because these two environments will affect the activity of the enzyme or even lose its effect. In addition, when the temperature is higher than 40°C, it will directly lose its characteristics. More importantly, because washing will affect the characteristics of the enzyme, the measurement of the enzyme type is all single use

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: Cleaning treatment of substrate components

[0028] The substrate assembly used in the present invention includes a paper-based chip and a silicon substrate. In order to prevent the substrate assembly from being affected by impurities on the surface of the substrate assembly when laying the seed layer, it needs to be cleaned first. Paper-based chips are handled differently from silicon substrates and are handled separately. Open the paper-based chip from the wrapping paper, take out the new paper-based chip, rinse it with deionized water, dry it with a nitrogen spray gun, and then place it in a clean transparent box to avoid re-contamination of the surface. The silicon substrate is soaked in acetone first, and cleaned with an ultrasonic oscillator for 10 minutes to remove the remaining oil stains on the silicon substrate. Remove the remaining organic matter, then take out the silicon substrate, then soak it in deionized water and shake for 10 minutes to r...

Embodiment 2

[0030] Embodiment 2: Preparation of seed solution and growth solution

[0031] image 3 Make a schematic diagram for the seed solution, which uses methanol as the solvent and sodium hydroxide and zinc acetate as the solute. Specifically, add 0.12 grams of sodium hydroxide to 100 ml of methanol to make a sodium hydroxide solution; add another 0.328 grams of zinc acetate to 150 ml of methanol to make a zinc acetate solution, and then stir the zinc acetate solution under electromagnetic heating After stirring in the container and heating to 60°C, the sodium hydroxide solution is then dripped into the zinc acetate solution at a rate of 50ml per hour by using a constant pressure delivery pump. After dripping 100ml, continue to heat at 60°C for three hours. After stirring for 10 hours, the solution turned from clear to turbid, and this solution was the seed solution of zinc oxide nanowires.

[0032] The growth solution is the solution needed in the hydrothermal method, and it is a...

Embodiment 3

[0040] Example 3: Add conductive ink

[0041] After the substrate assembly is cleaned, zinc oxide nanowire materials will be grown on the working electrode of the paper-based chip. There are three electrodes (working electrode, opposite electrode, and reference electrode) on the paper-based chip of the present invention, so heat-resistant glue is used. Cover a part of the paper-based chip, use a puncher to operate the substrate assembly, punch a hole with the paper-based chip, make the hole face the working electrode and stick it on the paper-based chip, and the area of ​​the hole is the same as the area of ​​the working electrode, only Let the working electrode be exposed, while the other two electrodes are covered, and then add conductive ink, drop into the seed solution, and soak in the growth solution.

[0042] The conductive ink is used to increase the conductivity between the nanowire and the working electrode and increase the current value for sensing the glucose concen...

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Abstract

The invention discloses a non-enzymatic glucose sensing detection system and a preparation process thereof. Zinc oxide nanowires with good quality and fewer defects can be manufactured by utilizing the simple and low-temperature hydrothermal process, a conductive ink layer which is additionally added and attached to a bottom layer enhances sensing capability of the zinc oxide nanowires so that thezinc oxide nanowires have good quality, good sensitivity and relatively high determination coefficient in physical property, the measurement range is better than that of detection paper, and more accurate glucose concentration within 3mMol / L can be measured.

Description

technical field [0001] The invention relates to the field of new materials, in particular to a non-enzymatic glucose sensing and detection system and a preparation process thereof. Background technique [0002] Glucose sensors can be classified into enzymatic and non-enzymatic types. The enzyme type is to oxidize glucose by using glucose oxidase, and then display the data with electrical signals through the sensor device, and then know the content; the non-enzyme type is to use electrochemical reactions to simulate the action of enzymes, and then oxidize glucose through electron movement The effect is displayed through the sensor system. [0003] Enzymatic glucose biosensors using glucose enzymes such as glucose oxidase have found that their chemical instability, operational complexity, and poor reproducibility are the main obstacles to their widespread application, and they cannot be used below pH2 and above pH8. Use, because these two environments will affect the activit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30B82Y40/00B82Y30/00
CPCB82Y30/00B82Y40/00G01N27/308G01N27/327
Inventor 陈英兰李娟
Owner 融创达科技成果转化(江苏)有限公司
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