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Method for producing polyhydroxyalkanoates by using acetic acid or butyric acid

A technology of polyhydroxyalkanoate and acetic acid, applied in the field of biochemistry, can solve the problems of restricting large-scale industrial production and application, high production cost of PHA, etc., achieves good industrial application prospects, reduces fermentation costs, and has great economic advantages Effect

Active Publication Date: 2020-05-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production cost of PHA is still high, which limits its large-scale industrial production and application.

Method used

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  • Method for producing polyhydroxyalkanoates by using acetic acid or butyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, utilize acetic acid to produce polyhydroxyalkanoate (shaking flask culture) with Neptunomonas

[0073] 1. Production of polyhydroxyalkanoate from acetic acid by Neptunonas bacterium

[0074] 1. Aseptically prepare Neptunonas seed solution

[0075] (1) Activation of bacteria

[0076] Take a glycerol tube of strains stored in a -80°C refrigerator, streak and inoculate it on a TYS medium plate, and incubate at 37°C for 12-24h.

[0077] (2) First-class seeds

[0078] Pick a single colony from the plate that has completed step (1), inoculate it in liquid TYS medium, and cultivate it at 37° C. with shaking at 200 rpm for 10-12 hours.

[0079] (3)Secondary seed

[0080] Take the primary seed liquid obtained in step (2), inoculate it in liquid TYS medium according to the inoculum amount of 1%, add 10 g / L acetic acid as carbon source, and culture at 37°C and 200 rpm for 10-12 hours with shaking.

[0081] 2. Prepare liquid TYS medium, add acetic acid to a final...

Embodiment 2

[0101] Embodiment 2, utilize butyric acid to produce polyhydroxyalkanoate (shake flask culture) with Neptunomonas

[0102] 1. Production of polyhydroxyalkanoate from butyric acid by Neptunomonas

[0103] 1. Aseptically prepare Neptunonas seed solution

[0104] (1) Activation of bacteria

[0105] Take a glycerol tube of strains stored in a -80°C refrigerator, streak and inoculate it on a TYS medium plate, and incubate at 37°C for 12-24h.

[0106] (2) First-class seeds

[0107] Pick a single colony from the plate that has completed step (1), inoculate it in liquid TYS medium, and cultivate it at 37° C. with shaking at 200 rpm for 10-12 hours.

[0108] (3)Secondary seed

[0109]Take the primary seed liquid obtained in step (2), inoculate it in liquid TYS medium according to the inoculum amount of 1%, add 10 g / L butyric acid as a carbon source, and culture at 37°C and 200 rpm for 10-12 hours with shaking.

[0110] 2. Prepare liquid TYS medium, add butyric acid to a final conc...

Embodiment 3

[0121] Embodiment 3, utilize butyric acid to produce polyhydroxyalkanoate (fermenter cultivation) with Neptunomonas

[0122] 1. Production of polyhydroxyalkanoate from butyric acid by Neptunomonas

[0123] 1. Aseptically prepare Neptunonas seed solution

[0124] (1) Activation of bacteria

[0125] Take a glycerol tube of strains stored in a -80°C refrigerator, streak and inoculate it on a TYS medium plate, and incubate at 37°C for 12-24h.

[0126] (2) First-class seeds

[0127] Pick a single colony from the plate that has completed step (1), inoculate it in liquid TYS medium, and cultivate it at 37° C. with shaking at 200 rpm for 10-12 hours.

[0128] (3)Secondary seed

[0129] Take the primary seed liquid obtained in step (2), inoculate it in liquid TYS medium according to the inoculum amount of 1%, add 10 g / L butyric acid as a carbon source, and culture at 37°C and 200 rpm for 10-12 hours with shaking. A total of 300ml of secondary seed liquid is required for fermenter ...

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Abstract

The invention discloses a method for producing polyhydroxyalkanoates by using acetic acid or butyric acid. According to the method, neptunomonas concharum with a polyhydroxyalkanoate protection capability is capable of accumulating a great number of polyhydroxyalkanoates by using acetic acid or butyric acid as a carbon source under open conditions without sterilization, the ratio of the polyestersto the dry weight of bacteria is as high as 69%, and the yield of the method with the butyric acid as the carbon source is up to 40% of a theoretic yield at most. By adopting the method, the two carbon sources, namely the acetic acid and the butyric acid, are adopted to produce the polyhydroxyalkanoates, no sterilization is needed in the fermentation process, the fermentation cost of the polyhydroxyalkanoates can be reduced, and great economic advantages and good industrial application prospects can be achieved.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to a method for producing polyhydroxyalkanoate, more specifically to a method for producing polyhydroxyalkanoate by using acetic acid or butyric acid. Background technique [0002] Polyhydroxyalkanoates (PHA for short) is a kind of high molecular polymer synthesized by microorganisms in cells under the condition of unbalanced growth and metabolism. Microorganisms decompose and use again. PHA has similar material properties to petroleum-derived plastic materials, and at the same time has excellent properties that many petroleum-derived plastic materials lack, such as biorenewability, biodegradability, biocompatibility, and so on. For many years, the synthesis and utilization of PHA has attracted extensive attention from the scientific and industrial circles. People have carried out a lot of research work on reducing the production cost of PHA, synthesizing PHA materials with new monomer co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/01
CPCC12P7/625
Inventor 李正军普楠史理陇陶观宝
Owner BEIJING UNIV OF CHEM TECH
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