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Method and device for extracting gene fusion immune treatment neoantigen by integrating deep sequencing data of DNA and RNA

A technology of gene fusion and data extraction, applied in genomics, sequence analysis, instruments, etc., can solve the problems of high false positives, achieve the effect of improving effectiveness, improving effect, and reducing false positive rate

Active Publication Date: 2020-05-22
深圳市新合生物医疗科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the identification of gene fusions based on a single data source (DNA or RNA) usually has high false positives, prediction of neoantigens generated by fusion requires more abundant data and a strict screening process to ensure the high efficiency of neoantigen vaccines

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  • Method and device for extracting gene fusion immune treatment neoantigen by integrating deep sequencing data of DNA and RNA
  • Method and device for extracting gene fusion immune treatment neoantigen by integrating deep sequencing data of DNA and RNA

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Embodiment Construction

[0045] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0046] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the following specific specific embodiments; it should also be understood that the terms used in the examples of the present invention are to describe specific specific embodiments, It is not intended to limit the protection scope of the present invention.

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Abstract

The invention discloses a method and a device for extracting a gene fusion immune treatment neoantigen by integrating deep sequencing data of DNA and RNA. The method comprises the following steps: S10, acquiring a genome gene fusion sequence of a sample; S20, acquiring a transcriptome gene fusion sequence of the sample; S30, constructing a gene fusion proteome; and S40, obtaining a sample neoantigen. The tumor specific neoantigens discovered by the scheme of the invention are all from gene fusion, so that the screening range of the neoantigens is expanded, and an ammunition library of an immune treatment method based on the neoantigens is enriched. Through analysis and integration of tumor sample whole exome sequencing data and transcriptome sequencing data, gene fusion events in tumor tissues are comprehensively detected, the false positive rate of neoantigens generated by fusion is reduced, the effectiveness of neoantigen vaccine is improved, and the method is of great significance to improvement of the clinical immune treatment effect.

Description

technical field [0001] The invention relates to the field of tumor immunotherapy, in particular to a method and a device for extracting neoantigens for gene fusion immunotherapy by integrating deep sequencing data of DNA and RNA. Background technique [0002] Therapeutic concepts and methods for malignant tumors have been deeply developed in the past few decades. Traditional tumor treatment methods include surgery, radiotherapy, radiotherapy, and targeted therapy based on mutation typing. However, the above treatments have certain limitations in terms of toxic side effects and drug resistance. In recent years, new breakthroughs have been made in the concept of immunotherapy that inhibits and kills tumor cells by activating the immune system. Existing immunotherapy methods can be divided into three categories according to their mechanism of action: (1). Immune checkpoint inhibitors that activate the immune system by inhibiting the inhibitory pathway of the immune system, (2)...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20G16B25/10G16B30/10
CPCG16B20/20G16B25/10G16B30/10
Inventor 万季潘有东汪健徐韵婉宋麒刘鹏夏迪
Owner 深圳市新合生物医疗科技有限公司
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