Method for separating and identifying bacteria in interlayer oxidation zone sandstone type uranium deposit

A technology of interlayer oxidation zone and identification method, which is applied in the field of bacteria separation and identification in the sandstone-type uranium deposit of the interlayer oxidation zone, can solve the problem that the relationship between the distribution of microorganisms and the geological and geochemical is poorly understood and other problems, and can reduce the ore prospecting rate. cost, high-efficiency bacterial culture technology, and the effect of reducing mining costs

Inactive Publication Date: 2020-05-22
BEIJING RES INST OF URANIUM GEOLOGY
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Problems solved by technology

[0002] At present, a large number of soil-born or stone-born microorganisms have been found in sediments and rocks, but little is known about the types and metabolic activities of microorganisms related to uranium deposits, and the relationship between the distribution of these microorganisms and geology and geochemistry

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Embodiment Construction

[0028] The present invention is described in further detail below in conjunction with embodiment.

[0029] The invention provides a method for separating and identifying bacteria in a sandstone-type uranium deposit in an interlayer oxidation zone. The method specifically includes the following steps:

[0030] Step (1) Determine the regional geological background of the study area, and confirm that there is indeed a certain amount of microorganisms in the ores of the Shihongtan uranium deposit in the Turpan-Hami Basin, and the microorganisms are closely related to uranium mineralization;

[0031] Step (2) isolation and observation of bacterial strains in the ore;

[0032] Step (2.1) Select 3 pieces of rock samples with 5 cm cracks and no cracks at the Shihongtan uranium deposit in the Turpan-Hami Basin;

[0033] Step (2.2) Wash the surface of the rock with sterile water until the water is clear, quickly burn it with alcohol to get the inner rock, and then store it in an airtig...

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Abstract

The invention belongs to the technical field of geology, and particularly relates to a method for separating and identifying bacteria in an interlayer oxidation zone sandstone type uranium deposit. The method specifically comprises the following steps: determining the geological background of a research area, and determining whether a large number of microorganisms exist in typical uranium deposituranium ore or not; separating and observing strains in the ore; and extracting total bacteria in the ore. According to the invention, through the in-situ separation of the interlayer oxidation zonesandstone type uranium deposit bacteria, the types of special bacteria in the special mineralization environment of the typical uranium deposit are determined, the characteristics of the bacteria areresearched, the types of the bacteria and the microorganisms with high uranium ore enrichment capacity can be further found out, the contribution of different microorganisms to sandstone uranium ore mineralization is determined, the uranium ore forming environment is further determined, uranium ore prospecting is guided, and the ore prospecting cost is reduced. Meanwhile, the method can provide afeasible bacterial population for in-situ leaching uranium mining, provides a feasible and efficient bacterial liquid culture technology for a microbial ore leaching process, and reduces the mining cost.

Description

technical field [0001] The invention belongs to the technical field of geology, and in particular relates to a method for separating and identifying bacteria in sandstone-type uranium deposits in interlayer oxidation zones. Background technique [0002] At present, a large number of soil-born or stone-born microorganisms have been found in sediments and rocks, but the types of microorganisms and their metabolic activities related to uranium deposits, as well as the relationship between the distribution of these microorganisms and geology and geochemistry are still poorly understood. It has been confirmed through previous experiments and exploration that a certain amount of microorganisms do exist in the ores of the Shihongtan uranium deposit in the Turpan-Hami Basin. [0003] Therefore, it is necessary to carry out simulation experiments on the relationship between microorganisms and uranium mineralization in sandstone-type uranium deposits in interlayer oxidation zones, est...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12Q1/6869C12Q1/04C12R1/01
CPCC12N1/20C12N1/02C12Q1/6869C12Q2531/113
Inventor 张玉燕李子颖蔡煜琦修晓茜王文全
Owner BEIJING RES INST OF URANIUM GEOLOGY
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