Rice sheath blight effector gene RsIA-NP8 and application thereof
A rice sheath blight fungus and gene technology, applied in the field of genetic engineering, can solve problems such as ineffective effects
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[0031] 1. Predict the isolation and cloning of effector genes
[0032] Under the ultra-clean workbench, use tweezers to pick a small amount of Rhizoctonia solani AG1IA cells, insert them into 50ml PDA medium, cultivate them at 28°C and 200r / m for two days, filter and collect mycelium with four layers of gauze, and use Total RNA was extracted by liquid nitrogen grinding. Using oligodT as a primer, cDNA was obtained by reverse transcription.
[0033] Primers were designed according to the predicted sequences, and the primer sequences are as follows:
[0034] Forward primer: 5'-ATGCGTTTTGCGACCGTAGC-3' (SEQ ID NO: 3);
[0035] Reverse primer: 5'-GATGGCAACGCACGATTGTT-3' (SEQ ID NO: 4).
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