Bifidobacterium animalis and application of compound bacterium preparation prepared from bifidobacterium animalis in preparation of medicine for treating or preventing avian influenza virus infection

A technology of animal bifidobacteria and avian influenza virus, which is applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc. Lost, improve survival rate, increase the effect of clearing

Active Publication Date: 2020-05-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although bifidobacteria have some reports on the prevention and treatment of intestinal infections, there are few reports on anti-intestinal infection as probiotics, especially in respiratory tract infections, and the antiviral effects of bifidobacteria on animals The effect has not been reported yet, and the present invention reports the application of Bifidobacterium animalis anti-influenza virus for the first time

Method used

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  • Bifidobacterium animalis and application of compound bacterium preparation prepared from bifidobacterium animalis in preparation of medicine for treating or preventing avian influenza virus infection
  • Bifidobacterium animalis and application of compound bacterium preparation prepared from bifidobacterium animalis in preparation of medicine for treating or preventing avian influenza virus infection
  • Bifidobacterium animalis and application of compound bacterium preparation prepared from bifidobacterium animalis in preparation of medicine for treating or preventing avian influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of the probiotic preparation of anti-H7N9 avian influenza virus infection:

[0040] 1. Preparation of single bacterial preparations of Bifidobacterium animalis and Bifidobacterium pseudolongum

[0041] Bifidobacterium animalis ATCC 25527 and Bifidobacterium pseudolongum ATCC 25526. Resuspend the lyophilized powder of the strain with sterile PBS, pick an appropriate amount of bacterial liquid with an inoculation loop, streak and rejuvenate it on the MRS solid plate medium, and culture it under anaerobic conditions at 37°C for 24-48 hours. Streak culture the rejuvenated Bifidobacterium animalis and Bifidobacterium pseudolongum in MRS respectively, and culture them under anaerobic conditions at 37°C for 24 hours, wash the bacterial lawn on the MRS medium plate with PBS, and collect the bacterial suspension , centrifuge at 4°C and 5000rpm for 10 minutes, discard the supernatant, resuspend the collected Bifidobacterium animalis and Bifidobacterium pse...

Embodiment 2

[0046] Effect of the probiotic preparation prepared in Example 1 on mouse mortality and body weight after H7N9 avian influenza virus infection

[0047] 1. Effects on Mortality and Body Weight of SPF Mice

[0048] The experimental animals were 8-week-old female C57BL / 6 specific pathogen-free (SPF) mice, a total of 80 were randomly divided into 4 groups, 20 in each group, and all animals were raised in a biosafety level 3 laboratory (ABSL3) . The first group is the control group fed with sterile PBS, 100ul per mouse; the second group is fed with Bifidobacterium pseudolongum (Bifidobacterium pseudolongum) ATCC 25526 single bacteria preparation group, 100ul per mouse; the third group is The single-bacteria preparation group of Bifidobacterium animalis ATCC 25527 was fed with 100ul per mouse; the fourth group was fed with the compound preparation of Bifidobacterium animalis ATCC 25527 and Bifidobacterium pseudolongum ATCC 25526 Group, 100ul per mouse.

[0049] Antibiotic treatme...

Embodiment 3

[0064] The effect of the probiotic preparation prepared in Example 1 on the influenza virus content in the lungs of mice after H7N9 avian influenza virus infection, the impact on the lung tissue structure of mice, and the impact on the cytokines in the lungs and the cytokines in the blood

[0065] 96 SPF mice were randomly divided into 4 groups, 24 in each group. The first group was fed with sterile PBS control group, 100ul per mouse; the second group was fed with Bifidobacterium pseudolongum (Bifidobacterium pseudolongum) ATCC 25526 single-bacteria preparation group, 100ul per mouse; the third group was fed with Bifidobacterium animalis (Bifidobacterium animalis) ATCC25527 single-bacteria preparation group, 100ul per mouse; the fourth group was fed with Bifidobacterium animalis ) ATCC 25527 and Bifidobacterium pseudolongum (Bifidobacterium pseudolongum) ATCC 25526 compound preparation group, 100ul per mouse.

[0066] As in Example 2, all SPF mice were raised in a biosafety le...

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Abstract

The invention relates to the field of biological preparations, and particularly discloses bifidobacterium animalis and application of a compound bacterium preparation prepared from the bifidobacteriumanimalis in preparation of a medicine for treating or preventing avian influenza virus infection. The invention discovers that the bifidobacterium animalis ATCC 25527 can be used for treating animalsinfected by H7N9, and after the bifidobacterium animalis ATCC 25527 is compounded with bifidobacterium pseudolongum ATCC 25526, the effect is better; the compound preparation can regulate the body immune response, remarkably improve weight loss and lung tissue injury caused by H7N9 influenza virus infection, and obviously increase the survival rate of mice infected by the H7N9 influenza virus. The compound probiotic preparation provided by the invention has a remarkable effect of resisting the H7N9 influenza virus infection, and can be effectively used for preventing and treating the avian influenza virus infection.

Description

technical field [0001] The invention relates to the field of biological preparations against influenza virus infection, in particular to the application of animal bifidobacteria and the compound bacteria preparation prepared therefrom in the preparation of medicines for treating or preventing avian influenza virus infection. Background technique [0002] Avian influenza virus belongs to the Orthomyxoviridae family Influenza A virus and is a highly contagious infectious disease worldwide. The disease mainly occurs in poultry and wild poultry, which seriously hinders the development of the poultry industry. At the same time, some of its subtypes (H5, H7, H9, H10) can also spread to humans across species, causing symptoms such as fever, cough, muscle aches, conjunctivitis, respiratory diseases, and even death in severe cases, causing serious harm to human health. serious threat. [0003] For a long time, avian influenza viruses have been relatively mild and only spread among ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/745A61P31/16
CPCA61K35/745A61P31/16
Inventor 金梅林张强胡晓通杨丽胡进王婷孙小美邹忠龚文孝林显
Owner HUAZHONG AGRI UNIV
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