Application of vitamin k3 in the preparation of antiallergic drugs

A vitamin and allergic reaction technology, which is applied in the field of antiallergic drug preparation, can solve the problems such as application reports and adverse reactions of antiallergic drugs that have not been seen, and achieves reduction of mast cell degranulation, improvement of symptoms, and relief of local and systemic allergic reactions. Effects of Allergy Symptoms

Active Publication Date: 2021-11-19
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current drugs for such diseases all act in the symptom control stage downstream of the allergic reaction mechanism, and the sensitization mediators released by mast cells still exist in the body and have potential toxicity
Long-term use of the above drugs can cause a variety of adverse reactions
[0005] At present, there is no report on the application of vitamin K3 as an anti-allergic drug

Method used

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  • Application of vitamin k3 in the preparation of antiallergic drugs
  • Application of vitamin k3 in the preparation of antiallergic drugs
  • Application of vitamin k3 in the preparation of antiallergic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Vitamin K3 inhibits SP-induced KU812 cell β-hexosaminidase release

[0025] 1. Experimental materials

[0026] SP (purchased from MCE Company), KU812 cells, RPMI1640 medium (containing penicillin 100 U / mL, streptomycin 0.1 mg / mL, 20% FBS), Tyrode's solution, vitamin K3 (purchased from Dr. Ehrenstorfer GmnH Company), 0.1 M Na 2 CO 3 , 0.1M NaHCO 3 , β-hexosaminidase, 0.1M citric acid, 0.1M sodium citrate.

[0027] The used SP (Substance P) refers to the neuropeptide discovered by Von Euler and Gaddum in 1931, the sequence is Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, and the molecular weight is 1340 .

[0028] Further, the purity of SP is ≥97%.

[0029] 2. Experimental method

[0030] KU812 cells were seeded in 96-well plates (1×105 cells / well), and the culture medium was discarded by centrifugation. Vitamin K3 solutions (concentrations of 0 μM, 50 μM, 100 μM, 200 μM and 400 μM) were prepared with sterile Tyrode’s solution, added to corresponding ...

Embodiment 2

[0034] Example 2 Vitamin K3 inhibits the release of MCP-1 in human KU812 cells caused by SP

[0035] 1. Experimental materials

[0036] SP (purchased from MCE Company), KU812 cells, RPMI1640 medium (containing penicillin 100U / mL, streptomycin 0.1 mg / mL, 20% FBS), Tyrode's solution, vitamin K3 (purchased from Dr. Ehrenstorfer GmnH Company), human MCP-1ELISA kit (purchased from Beijing Yiqiao Shenzhou Company)

[0037] The used SP (Substance P) refers to the neuropeptide discovered by Von Euler and Gaddum in 1931, the sequence is Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, and the molecular weight is 1340 .

[0038] Further, the purity of SP is ≥97%.

[0039] 2. Experimental method

[0040] KU812 cells were seeded in 96-well plates (1×105 cells / well), and the culture medium was discarded by centrifugation. KU812 cell solutions were prepared with sterile Tyrode's solution (concentrations were 0 μM, 12.5 μM, 25 μM, 50 μM, respectively), added to corresponding well plates...

Embodiment 3

[0044] Example 3 Vitamin K3 inhibits mouse toe swelling and exudation caused by SP

[0045] 1. Experimental materials

[0046] SP (purchased from MCE Company), 6-8 week-old male C57 mice (purchased from the Experimental Animal Center of Xi'an Jiaotong University), vitamin K3 (purchased from Dr. Ehrenstorfer GmnH Company), 0.4% Evans blue solution, 3.5% chlorine hydrate Aldehyde solution, PBS solution, saline, acetone.

[0047] 2. Experimental method

[0048] The mice were divided into blank control group, positive control group and vitamin K3 administration groups of various concentrations. Vitamin K3 solutions of different concentrations (0.75 mg / kg, 1.5 mg / kg, 3 mg / kg respectively) were prepared with physiological saline for intraperitoneal injection. After 30 minutes, mice were anesthetized with 3.5% chloral hydrate solution, and 0.4% Evans blue solution was injected into the tail vein. The thickness of the left and right soles of the mice in each group was measured, an...

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PUM

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Abstract

The invention discloses the application of vitamin K3 in the preparation of anti-allergic drugs. Vitamin K3 has a clear therapeutic effect on improving anaphylactoid symptoms. By comparing the degranulation of KU812 cells, the degree of swelling of the toe, and the degree of Evans blue exudation of the toe , Serum histamine release evaluation test drug group, normal saline control group and normal control group, to evaluate the therapeutic effect of drugs on allergic diseases, to determine that vitamin K3 can be used in the preparation of anti-allergic drugs. Vitamin K3 can effectively alleviate the symptoms of local and systemic anaphylaxis in mice; reduce mast cell degranulation and significantly improve the symptoms caused by anaphylaxis.

Description

technical field [0001] The invention belongs to the field of preparation of antiallergic drugs, and relates to the application of vitamin K3 in the preparation of antiallergic drugs. Background technique [0002] Vitamin K3 (2-methyl-1,4-naphthoquinone) is a fat-soluble vitamin that participates in the synthesis of coagulation factors and other substances in the liver. As a good hemostatic drug, its main function is to participate in the synthesis of thrombin, promote blood coagulation, can effectively prevent and treat bleeding diseases, and also participate in the mineralization of bones. Vitamin K3 is also an important component of feed additives, an indispensable nutrient for the growth and development of poultry and livestock, and can also be used as plant growth regulators, accelerators, herbicides, etc. At present, it is often used in the form of injection and oral dripping pills for the treatment of clinical bleeding disorders. [0003] Anaphylaxis is not an immune...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A61P37/08
CPCA61K31/122A61P37/08
Inventor 王楠贺浪冲吴媛媛王程
Owner XI AN JIAOTONG UNIV
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