Establishment method and detection method of uplc characteristic map of Tiankuizi medicinal material

A technology of characteristic map and establishment method, which is applied in the field of establishment method and detection of UPLC characteristic map of Tiankuizi medicinal material, can solve the problems of lack of quantitative index and overall quality control method of Tiankuizi medicinal material, and facilitate large-scale promotion application, high precision and easy operation

Active Publication Date: 2022-03-11
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 2015 edition of the Chinese Pharmacopoeia lacks the quantitative indicators and the overall quality control method of geranium seeds, and there are few research reports on the quality control standards of geranium seeds, it is necessary to establish a comprehensive and reliable detection method to evaluate the The quality of sub-medicinal materials is very necessary

Method used

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  • Establishment method and detection method of uplc characteristic map of Tiankuizi medicinal material
  • Establishment method and detection method of uplc characteristic map of Tiankuizi medicinal material
  • Establishment method and detection method of uplc characteristic map of Tiankuizi medicinal material

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Embodiment 1

[0061] This embodiment is a method for establishing the UPLC characteristic map of the medicinal material of Radix Geloniae Sinensis.

[0062] Tiankuizi medicinal materials: Collect 15 batches of Tiankuizi medicinal materials from Guangdong, Guangxi, Hunan, Hubei, Chongqing and other places of origin that meet the requirements of the Chinese Pharmacopoeia in 2015.

[0063] The establishment method is as follows:

[0064] 1. Chromatographic conditions and system suitability test

[0065] Use octadecylsilane bonded silica gel as filler (Waters ACQUITY HSS T3, column length is 150mm, inner diameter is 2.1mm, particle size is 1.8μm); methanol is used as mobile phase A, and 0.05% phosphoric acid solution is used as mobile phase B. Carry out gradient elution according to the regulations in Table 1; the flow rate is 0.25ml per minute; the column temperature is 15°C; the detection wavelength is 260nm. The number of theoretical plates should not be less than 30,000 based on the peak ...

Embodiment 2

[0092] This embodiment is a method for detecting the contents of the index components of the medicinal material of Radix Geranium.

[0093] Tiankuizi has the effects of clearing heat and detoxifying, reducing swelling and dissipating stagnation, etc. It is commonly used clinically for carbuncles, boils, mastitis, scrofula, and snake bites. Among them, lactones, nitro and cyano have biological activities such as antibacterial, anti-inflammatory, and anti-oxidation, and alkaloids are commonly used effective pharmacological ingredients for anti-tumor. In the invention, the glipefrolactone and magnolanine are used as content determination indexes to evaluate the geranium medicinal material.

[0094] The detection method is as follows:

[0095] 1. Chromatographic conditions and system suitability test

[0096] Use octadecylsilane bonded silica gel as filler (Waters ACQUITY HSS T3, column length is 150mm, inner diameter is 2.1mm, particle size is 1.8μm); methanol is used as mobile...

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Abstract

The invention relates to a method for establishing a UPLC characteristic map and a detection method of a geranium medicinal material. The establishment method comprises the following steps: preparation of reference substance solution: taking glipifrolactone reference substance, shikonin reference substance and magnolialine reference substance, adding solvent and dissolving to obtain reference substance solution; preparation of test solution : get geranium medicinal material powder, add solvent and extract, extract filter, get continued filtrate, obtain need testing solution; Inject described reference substance solution and need testing solution into ultra-high performance liquid chromatograph and detect, all The mobile phase A adopted by the ultra-high performance liquid chromatograph is methanol, the mobile phase B is an aqueous phosphoric acid solution with a volume fraction of 0.03-0.07%, and the elution method is gradient elution. The establishment method can obtain the characteristic spectrum of the medicinal material of Tiankuizi, which contains 12 characteristic peaks. In addition, the content of glipifrolactone and magnolanine in the geranium seed medicinal material can also be determined simultaneously, which can comprehensively reflect the quality of the geranium seed medicinal material, and the method is accurate, reliable, easy to operate and good in reproducibility.

Description

technical field [0001] The invention relates to quality detection of traditional Chinese medicinal materials, in particular to a method for establishing a UPLC characteristic map and a detection method of the medicinal material of Tiankuizi. Background technique [0002] The 2015 edition of the Chinese Pharmacopoeia recorded geranium seeds, the source of which is the dried roots of the Ranunculaceae plant genus Semiaquilegia adoxoides (DC.) Makino, excavated in early summer, washed, dried, and removed from the fibrous roots. The original plant geranium is aliased as geranium, mouse dung grass, mouse dung or purple-backed geranium, and geranium is also known as thousand-year mouse dung, Tianquzi, didingzi, golden mouse dung, summer without trace or purple back Geranium root. Tiankuizi first appeared in Zhao Xuemin's "Compendium of Materia Medica Supplements" in the Qing Dynasty. [0003] The main chemical components of tiankui seeds are lactones, nitro and cyano groups, alk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 刘晓霞丁青陈芳梁月仪王利伟陈向东孙冬梅魏梅程学仁
Owner GUANGDONG YIFANG PHARMA
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