UPLC specific chromatogram establishing method and detection method of radix semiaquilegiae medicinal material
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A technology of characteristic map and establishment method, which is applied in the field of establishment method and detection of UPLC characteristic map of Tiankuizi medicinal material, can solve the problems of lack of quantitative index and overall quality control method of Tiankuizi medicinal material, and facilitate large-scale promotion Application, reliable results, and reproducible results
Active Publication Date: 2020-04-28
GUANGDONG YIFANG PHARMA
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Since the 2015 edition of the Chinese Pharmacopoeia lacks the quantitative indicators and the overall quality control method of geranium seeds, and there are few research reports on the qua
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Embodiment 1
[0061] This embodiment is a method for establishing the UPLC characteristic map of the medicinal material of Radix Geloniae Sinensis.
[0062] Tiankuizi medicinal materials: Collect 15 batches of Tiankuizi medicinal materials from Guangdong, Guangxi, Hunan, Hubei, Chongqing and other places of origin that meet the requirements of the Chinese Pharmacopoeia in 2015.
[0063] The establishment method is as follows:
[0064] 1. Chromatographic conditions and system suitability test
[0065] Use octadecylsilane bonded silica gel as filler (Waters ACQUITY HSS T3, column length is 150mm, inner diameter is 2.1mm, particle size is 1.8μm); methanol is used as mobile phase A, and 0.05% phosphoric acid solution is used as mobile phase B. Carry out gradient elution according to the regulations in Table 1; the flow rate is 0.25ml per minute; the column temperature is 15°C; the detection wavelength is 260nm. The number of theoretical plates should not be less than 30,000 based on the peak ...
Embodiment 2
[0092] This embodiment is a method for detecting the contents of the index components of the medicinal material of Radix Geranium.
[0093] Tiankuizi has the effects of clearing heat and detoxifying, reducing swelling and dissipating stagnation, etc. It is commonly used clinically for carbuncles, boils, mastitis, scrofula, and snake bites. Among them, lactones, nitro and cyano have biological activities such as antibacterial, anti-inflammatory, and anti-oxidation, and alkaloids are commonly used effective pharmacological ingredients for anti-tumor. In the invention, the glipefrolactone and magnolanine are used as content determination indexes to evaluate the geranium medicinal material.
[0094] The detection method is as follows:
[0095] 1. Chromatographic conditions and system suitability test
[0096] Use octadecylsilane bonded silica gel as filler (Waters ACQUITY HSS T3, column length is 150mm, inner diameter is 2.1mm, particle size is 1.8μm); methanol is used as mobile...
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Abstract
The invention relates to a UPLC specific chromatogram establishment method and a detection method of a radix semiaquilegiae medicinal material. The establishment method comprises the following steps:preparing a reference substance solution: taking a griffonilide reference substance, a lithospermoside reference substance and a magnoflorine reference substance, and adding a solvent for dissolutionto obtain a reference substance solution; preparing a test solution: taking a radix semiaquilegiae medicinal material powder, adding the solvent for extraction, filtering an extracting solution, and taking subsequent filtrate to obtain the test solution; and injecting the reference substance solution and the test solution into an ultra-high performance liquid chromatograph for detection, wherein amobile phase A adopted by the ultra-high performance liquid chromatograph is methanol, a mobile phase B adopted by the ultra-high performance liquid chromatograph is a phosphoric acid aqueous solution with a volume fraction of 0.03%-0.07%, and an elution mode is gradient elution. The establishing method can be used to obtain the specific chromatogram of the radix semiaquilegiae medicinal material, wherein 12 characteristic peaks are included. Besides, contents of griffonilide and magnoflorine in the radix semiaquilegiae medicinal material can be measured at the same time, the quality of the radix semiaquilegiae medicinal material can be comprehensively reflected, and the method is accurate, reliable, easy and convenient to operate and good in reproducibility.
Description
technical field [0001] The invention relates to quality detection of traditional Chinese medicinal materials, in particular to a method for establishing a UPLC characteristic map and a detection method of the medicinal material of Tiankuizi. Background technique [0002] The 2015 edition of the Chinese Pharmacopoeia recorded geranium seeds, the source of which is the dried roots of the Ranunculaceae plant genus Semiaquilegia adoxoides (DC.) Makino, excavated in early summer, washed, dried, and removed from the fibrous roots. The original plant geranium is aliased as geranium, mouse dung grass, mouse dung or purple-backed geranium, and geranium is also known as thousand-year mouse dung, Tianquzi, didingzi, golden mouse dung, summer without trace or purple back Geranium root. Tiankuizi first appeared in Zhao Xuemin's "Compendium of Materia Medica Supplements" in the Qing Dynasty. [0003] The main chemical components of tiankui seeds are lactones, nitro and cyano groups, alk...
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