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Blood vessel organoid, methods of producing and using said organoids

A technology of organoids and blood vessels, applied in biochemical equipment and methods, vascular endothelial cells, cardiovascular system diseases, etc., can solve problems such as lack of similarity in vascular networks

Pending Publication Date: 2020-04-24
IMBA INSTITUT FUR MOLEKULARE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Previous vascular models still lack sufficient resemblance to naturally occurring in vivo vascular networks, and improved, more realistic vascular models are needed

Method used

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  • Blood vessel organoid, methods of producing and using said organoids
  • Blood vessel organoid, methods of producing and using said organoids
  • Blood vessel organoid, methods of producing and using said organoids

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Embodiment Construction

[0024] The present invention provides methods for generating artificial vascular organoids. These artificial organoids are cultured in vitro, but have a highly similar structure to capillaries in vivo. Organoids are miniaturized and simplified forms of organs produced in vitro in three dimensions that display true microscopic anatomy. They are derived from one or several cells from a tissue, embryonic stem cells or induced pluripotent stem cells, which can self-organize in three-dimensional culture due to their self-renewal and differentiation capacity.

[0025] The inventive organoids derived from human stem cells recapitulate the structure and function of human blood vessels. 3D vascular organoids from embryos and induced pluripotent stem cells are provided. These vascular organoids contain endothelium, perivascular pericytes, and basement membrane, and self-assemble into a luminal network of interconnected capillaries. Human vascular organoids transplanted into mice form...

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Abstract

The present invention relates to a method of generating an artificial blood vessel organoid, comprising providing stem cells capable of vascular differentiation, stimulating mesoderm differentiation in said stem cells, stimulating vascular differentiation in said stem cells, developing a cell aggregate from said stem cells, embedding said cell aggregates in a collagenous 3D matrix and stimulatingvascular differentiation of the aggregate in said collagenous 3D matrix; organoids obtainable from said method, uses of said methods and organoids in manipulation and screening studied and kits for performing the methods.

Description

[0001] The present invention relates to the field of artificial vascular organoids. [0002] Background of the invention [0003] Blood vessels are prone to various diseases called vascular diseases. Conditions in the vascular network can cause a range of health problems that can be serious or prove fatal. Such diseases may be due to environmental pathogenesis or due to developmental defects. [0004] Goodwin (Microvasc Res. 2007; 74(2-3):172-183) described an in vitro angiogenesis assay to assess the activity of agents affecting angiogenesis in the pathogenesis of many diseases. [0005] Duffy et al. (European Cells and Materials 21, 2011:15-30) describe the in vitro vascularization of collagen-glycosaminoglycan scaffolds in surface-adhered 2D cultures. [0006] Nakagami et al. (Hypertension 2006; 48:112-119) provided a method for vascularization through cell-matrix interactions using Matrigel, which forces embryonic stem cells into sprouting sprouts containing endothelium a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071A01K67/027A61K35/44A61K49/00A61K45/00A61P9/00A61P9/10A61P3/10A61P13/12A61P27/02A61P17/02
CPCC12N5/0691C12N2501/115C12N2501/155C12N2501/165C12N2501/415C12N2501/999C12N2506/02C12N2506/45C12N2533/54C12N2533/90A61K35/545A61P9/10A61P9/00A61K45/06
Inventor J.佩宁格R.威默D.克尔雅施基
Owner IMBA INSTITUT FUR MOLEKULARE BIOTECH
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