GN formaldehyde-free rapid stationary liquid and preparation method

Abstract

The invention relates to a GN formaldehyde-free rapid stationary liquid. The GN formaldehyde-free rapid stationary liquid is prepared from the following components in parts by volume: 60 to 80 percentof absolute ethyl alcohol, 10 to 20 percent of glacial acetic acid, 5 to 10 percent of glycerol, 5 to 10 percent of laurocapram and 0.3g / 100ml to 1g / 100ml of potassium sorbate. The beneficial effectsare that compared with the traditional formalin stationary liquid, all the components of the GN formaldehyde-free rapid stationary liquid are non-toxic or low-toxic substances; the GN formaldehyde-free rapid stationary liquid is commonly used in daily life, mild in smell, small in irritation to mucous membranes, high in fixing speed and capable of preserving the tissue form more completely, and has the advantages of more complete tissue antigen preservation, excellent fixing effect, easy coloring of tissue slices, clear cell contour of the tissue slices, easily available raw materials, simplepreparation method, no need of heating and other special conditions and low cost. The invention also provides a preparation method of the GN formaldehyde-free rapid stationary liquid.

Description

technical field [0001] The invention relates to a fixative solution for animal pathological examination and histomorphological research, in particular to a GN aldehyde-free fast fixative solution and a preparation method thereof. Background technique [0002] At present, the commonly used fixatives for animal tissues include formalin fixative, Bouin's fixative, aldose calcium fixative, AF fixative, etc. Most fixatives contain formaldehyde. Of all the fixatives available, formalin is the most commonly used fixative. It has strong penetration ability to tissue, uniform fixation, and less tissue shrinkage. Although it has high toxicity, it is still widely accepted by people because of its low price and better preservation of tissue structure, and it has been used as a common tissue fixative in pathology departments. But formaldehyde has following shortcoming: one, formaldehyde is volatile, has stronger irritant and corrosivity, seriously causes air and environmental pollutio...

AI Technical Summary

Problems solved by technology

But formaldehyde has the following disadvantages: one, formaldehyde is volatile, has stronger irritation and corrosiveness, seriously causes air and environmental pollution, and it is extremely difficult to protect

Second, if formaldehyde is stored for a long time, it will easily produce white precipitate, that is, paraformaldehyde, which will affect the fixation effect of tissue samples.

Third, formaldehyde is easily oxidized to form formic acid, making the solution acidic, resulting in acidification of the specimen, and in severe cases, affecting the staining effect of the nucleus

Fourth, long-term fixation of tissues affects the staining effect: old tissues fixed with formaldehyde, such as spleen and liver, are prone to form formaldehyde pigments in the tissues, which are easily confused with hemosiderin, which seriously affects the pathological diagnosis.

Fifth, formaldehyde waste liquid cannot be directly discharged into the sewer pipe, it needs special treatment, and the treatment cost is relatively high

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