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A new method of phase-shift phase microscopy based on f-p cavity

A technology of phase microscopy and a new method, which is applied in the direction of instruments, measuring devices, optical devices, etc., and can solve problems such as complex devices and complex devices

Active Publication Date: 2021-06-08
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Patent CN201710518263.5 discloses a three-refractive index tomographic microscopic imaging system and its method. Although it can restore the three-dimensional refractive index information of the sample, it is essentially different from the present invention in the imaging optical path, and the required devices more complicated
[0012] Patent CN201710904860.1 discloses an optical coherence tomography imaging system, which uses Mach-Zehnder interference optical path, which is characterized by the use of optical fibers to simplify the system and reduce costs, but compared with the optical path structure of the F-P cavity, it is still relatively complex

Method used

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  • A new method of phase-shift phase microscopy based on f-p cavity
  • A new method of phase-shift phase microscopy based on f-p cavity
  • A new method of phase-shift phase microscopy based on f-p cavity

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Embodiment 1

[0064] Figure 2b An embodiment of digital holographic recording optical path based on F-P cavity is given. The optical path includes a light source 21 , an attenuator 22 , a beam expander 23 , an F-P etalon 24 , a microstructured optical fiber 25 , a PZT26 , a microscope objective lens 27 , a CCD28 and a computer 29 . The light beam starts from the light source 21, passes through the attenuator 22, and the energy of the light beam weakens, the light beam passes through the beam expander 23, and the diameter of the light beam expands, and when the light beam passes through the F-P etalon 24 with the microstructured optical fiber 25, it is repeatedly reflected at the F-P etalon 24, After passing through the microstructured optical fiber 25 many times, each beam of transmitted light passing through the F-P etalon 24 passes through the microscope objective lens 27, and then interferes and superimposes on the CCD 28. The digital hologram of the interference is recorded by the CCD ...

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Abstract

The invention provides a new method of phase-shift phase microscopy imaging based on an F-P cavity. It is characterized in that it includes digital hologram recording based on F-P cavity, calculation of phase distribution by phase shift method, unwrapping, three-dimensional phase distribution reconstruction and three-dimensional refractive index conversion. The present invention mainly provides a new method of phase-shift phase microscopic imaging based on F-P cavity, which has higher sensitivity than the traditional microscopic imaging method. The invention has the advantages of simple structure, high sensitivity and accurate measurement. The invention can be used for high-resolution three-dimensional microscopic imaging of optically transparent objects, and can be widely used in non-destructive, label-free and non-contact three-dimensional tomographic imaging of tiny organisms and the like.

Description

[0001] (1) Technical field [0002] The invention relates to a new method of phase-shift phase microscopic imaging based on F-P cavity, which can be used for high-resolution three-dimensional microscopic imaging of optically transparent objects, and can be widely used in the non-destructive, label-free and non-contact microscopic imaging of tiny organisms. Three-dimensional tomography and the like belong to the technical field of microscopic imaging. [0003] (2) Background technology [0004] The three-dimensional refractive index distribution of the sample to be tested is an important inherent property. For the optically transparent sample to be tested, the three-dimensional refractive index distribution can reflect the microstructure, density and other information of the sample. Labeled, non-contact 3D tomography. [0005] In contemporary life science research, fluorescence imaging is usually used to label the samples to be tested. However, in the process of marking, it wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G03H1/00G03H1/04G03H1/22G01B9/021
CPCG01B9/021G03H1/0005G03H1/0443G03H1/22G03H2001/0038G03H2001/005
Inventor 苑立波孟令知李晟
Owner GUILIN UNIV OF ELECTRONIC TECH
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