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Separation tube for preparing sample for detecting content of toltrazuril in blood and detection method

A technology of toltrazuril and a detection method, which is applied to a separation tube for sample preparation in blood toltrazuril content detection and the field of detection, can solve the problems of low efficiency, cumbersome experimental steps and the like, and achieves high experimental efficiency, simple operation, The effect of protecting the instrument

Inactive Publication Date: 2020-04-17
镇江益生堂水产生物有限公司 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The experimental steps are cumbersome and the efficiency is low

Method used

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  • Separation tube for preparing sample for detecting content of toltrazuril in blood and detection method
  • Separation tube for preparing sample for detecting content of toltrazuril in blood and detection method
  • Separation tube for preparing sample for detecting content of toltrazuril in blood and detection method

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Embodiment

[0021] Separation tube for sample preparation for detection of toltrazuril content in blood, the separation tube is composed of an inner cylinder 3 and an outer cylinder 2, the inner cylinder 3 has upper and lower openings, and the inner cylinder 3 The nozzle of the outer cylinder extends into the outer cylinder 2, and the annular cavity formed between the inner and outer cylinders is the separation chamber 3, the bottom of the separation chamber 3 is provided with a filter packing layer 5, and the top of the separation chamber is provided with an inlet Liquid port 1; said inner cylinder is provided with a liquid-lifting pipe 5, said liquid-lifting pipe 5 extends into the outer cylinder 2 from the nozzle of the inner cylinder, and the height of said liquid-lifting pipe 5 is substantially equal to The height from the top of the 3 nozzles of the inner cylinder to the bottom wall of the outer cylinder 1; the shape of the 8 nozzles of the liquid-lifting pipe matches the internal sh...

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Abstract

The invention discloses a separation pipe for preparing a sample for detecting the content of toltrazuril in blood. The separation pipe is composed of an inner cylinder and an outer cylinder, whereinan upper portion and a lower portion of the inner cylinder are open, the inner cylinder extends into the outer cylinder from a pipe opening of the outer cylinder, an annular cavity formed between theinner cylinder and the outer cylinder is a separation cavity, a filtering filler layer is arranged at the bottom of the separation cavity, a liquid extraction pipe is arranged in the inner cylinder body and extends into the outer cylinder body from a pipe opening of the inner cylinder, a sealing gasket is arranged between the opening of the liquid extraction pipe and the opening of the inner cylinder, the liquid extraction pipe is also provided with an air guide pipe, the bottom of the air guide pipe is connected with an air guide port which is 10-20mm away from a bottom wall, the top of the gas guide pipe is communicated with an air cavity at an upper part of the inner cylinder, and a filter film is arranged on the bottom wall of the liquid extraction pipe. The invention also discloses amethod for detecting the content of toltrazuril in the blood by using high performance liquid chromatography after the blood is treated into a sample to be analyzed by using the separation pipe, and the method for detecting the content of toltrazuril in blood has advantages of simple operation, few steps and high experiment efficiency.

Description

technical field [0001] The invention relates to a method for detecting the blood content of medicines, in particular to a separation tube for sample preparation in the detection of blood toltrazuril content and a detection method. Background technique [0002] Toltrazuril is a triazine anticoccidial drug developed by Bayer in Germany in the 1980s. The drug can kill a variety of protozoa including coccidia, and has a wide anti-insect spectrum. Toltrazuril is mainly metabolized into toltrazuril sulfone and toltrazuril sulfone in the body. Toltrazuril and its metabolites have a particularly long half-life in animals, coupled with the extensive use of drugs and irrational drug use, it is easy to cause drug residues in animal foods and endanger food safety. Both the Ministry of Agriculture of my country and the European Union have made regulations on the residues of toltrazuril in various tissues of chicken, turkey and pigs, and toltrazuril sulfone is the residue marker. The t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14B01D36/00
CPCB01D36/00G01N30/02G01N30/14G01N2030/146
Inventor 黄文江贺文庆戴荣贵曹超江俊
Owner 镇江益生堂水产生物有限公司
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