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A kind of rabbit milk active small peptide solution and its extraction method and application

A technology of active small peptide and solution, which is applied in the field of rabbit milk active small peptide solution and its extraction, can solve the problems that the function of active small peptide has not been reported, achieve the safety and effectiveness of clinical application, promote the growth and stability of skin and nerves good effect

Active Publication Date: 2021-07-27
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function of active small peptides in rabbit milk has not been reported so far.

Method used

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  • A kind of rabbit milk active small peptide solution and its extraction method and application
  • A kind of rabbit milk active small peptide solution and its extraction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Take fresh rabbit milk, add an equal volume of 5% dilute sodium chloride aqueous solution under continuous stirring, and stir to mix evenly;

[0026] 2) After the salt precipitation reaction is completed, freeze the above solution at a temperature below -80°C for 1 hour, and then slowly melt it at a temperature not exceeding 40°C; Centrifuge in a centrifuge and collect the supernatant.

[0027] 3) Use a hollow fiber column ultrafilter to cut off components with a molecular weight less than 1KDa from the collected supernatant, and the working pressure is not higher than 0.1Mpa, and then perform reverse osmosis concentration on the obtained ultrafiltrate to obtain a concentrated solution for future use .

[0028] 5) Add 2% vitamin C by mass of the concentrated solution to the obtained concentrated solution to obtain a rabbit milk active small peptide solution.

[0029] It has been determined that the rabbit milk small peptide solution prepared in this example include...

Embodiment 2

[0031] 1) Take fresh rabbit milk, add an equal volume of 2% dilute sodium chloride aqueous solution under constant stirring, and stir to mix evenly;

[0032] 2) After the salt precipitation reaction is completed, freeze the above solution at -20°C for 5 hours, and then slowly melt it at a temperature not exceeding 40°C; Centrifuge in a centrifuge and collect the supernatant.

[0033] 3) Use a hollow fiber column ultrafilter to cut off components with a molecular weight less than 1KDa in the collected supernatant, and the working pressure is not higher than 0.2Mpa, and then perform reverse osmosis concentration on the obtained ultrafiltrate to obtain a concentrated solution for future use .

[0034] 5) Add 2% vitamin C by mass of the concentrated solution to the obtained concentrated solution to obtain a rabbit milk active small peptide solution.

[0035] It has been determined that the rabbit milk small peptide solution prepared in this example includes 30% organic substance...

Embodiment 3

[0037] 1) Take fresh rabbit milk, add an equal volume of aqueous solution containing 2.5% dilute sodium chloride under constant stirring, and stir to mix evenly;

[0038] 2) After the salt precipitation reaction is completed, freeze the above solution at a temperature below -40°C for 3 hours, and then slowly melt it at a temperature not exceeding 40°C; Centrifuge in a centrifuge and collect the supernatant.

[0039] 3) Use a hollow fiber column ultrafilter to cut off components with a molecular weight less than 1KDa from the collected supernatant, and the working pressure is not higher than 0.15Mpa, and then perform reverse osmosis concentration on the obtained ultrafiltrate to obtain a concentrated solution for future use .

[0040] 5) Add 3% vitamin C by mass of the concentrated solution to the obtained concentrated solution to obtain a rabbit milk active small peptide solution.

[0041] It has been determined that the rabbit milk small peptide solution prepared in this exam...

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Abstract

The invention discloses a rabbit milk active small peptide solution and its extraction method and application. The relative molecular weight range of the rabbit milk active small peptide is 300~1000Da, and the total small peptide content is not less than 1.2mg / mL. The rabbit milk of the present invention The active small peptide has biological characteristics, has good affinity with the tissue around the wound, can promote the growth of skin and nerves, facilitates the proliferation and repair of epithelial cells, can significantly improve the condition of wound granulation tissue, accelerate wound healing, and shorten healing time; and The molecular weight is low, the skin has a good absorption effect on it, and can quickly synthesize its own collagen, thereby forming normal connective tissue, filling and repairing damaged skin, and reducing the formation of scars. Therefore, the active small peptide of rabbit milk The solution is suitable for the treatment of various refractory wounds. The invention adopts low-temperature extraction, and the prepared rabbit milk active small peptide solution has stable quality, high activity, less impurities, is safe and effective in clinical application, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological products, in particular to a rabbit milk active small peptide solution and an extraction method and application thereof. Background technique [0002] Refractory wounds mainly refer to large-area skin and soft tissue defects or chronic wounds caused by severe trauma, which are characterized by large wound area, deep depth, chronic wound extracellular matrix remodeling, inhibited re-epithelialization, and long-term inflammatory response. Wound ulceration and infection, causing difficulty in healing, are among the problems frequently encountered in surgical and orthopedic surgery. The existing clinical medicines for surgical infection mainly include antibiotics and traditional Chinese medicines. Taking antibiotics has certain requirements on the dose and course of treatment, and the taking time should not be too long, and the side effects will be large. Shengji ointment is a commonly used medic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01A61K9/08A61P17/02C07K1/14C07K1/30C07K1/34C07K1/36
CPCA61K9/08A61K38/018A61P17/02C07K1/145C07K1/30C07K1/34C07K1/36
Inventor 王建华
Owner CHONGQING UNIV
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