Pear tissue culture seedling rooting and domestication method

A technology for tissue culture and tissue culture seedlings, which is applied in the directions of culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of low survival rate of transplanting rooted seedlings, low rooting rate of rooting formula, difficulty in rooting of tissue cultured seedlings, etc. , to achieve the effect of improving the survival rate of transplanting, enhancing photosynthesis, and increasing height

Inactive Publication Date: 2020-04-14
NORTHWEST A & F UNIV
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Problems solved by technology

The in vitro rapid propagation of Pyrus plants has been widely used, but there are still many shortcomings: pear is a taproot plant, and it is difficult for tissue cultured seedlings to take root. When transplanting, the tissue-cultured seedlings are directly transferred to the substrate for culti

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  • Pear tissue culture seedling rooting and domestication method

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Embodiment Construction

[0028] The method is to use a new formula to carry out rooting culture on the tissue-cultured seedlings, and then domesticate the rooted seedlings, thereby improving the transplanting survival rate of the tissue-cultured seedlings of the genus Pyrus, and the specific steps are as follows:

[0029] 1) rooting culture;

[0030] 1.1) After 4 weeks of subculture, select the tissue culture seedlings with strong growth potential (3cm or more in height, 0.4mm or more in stem diameter, dark green leaves, no necrosis in growth points, and no wilting in leaves), and cut off the callus and tissue in the lower part of the plant. For the extra leaves, keep the upper part of the plant about 2-3cm, and move it into the culture medium;

[0031] 1.2) According to different varieties, choose suitable auxin concentration and combination for cultivation, suitable auxin can promote rooting of plants; taking Pyrus Korla pear as an example, the suitable medium formula is: 1 / 2MS+1.5mg / L, IAA+0.5mg / ...

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Abstract

The present invention relates to a pear tissue culture seedling rooting and domestication method. The method comprises the following steps: 1) rooting culture is conducted: 1.1) after 4 weeks of subculture, strong growing tissue culture seedlings are selected, callus and excess leaves of lower parts of plants are removed, upper half parts of the plants are retained for about 2-3 cm, and the plantsare transferred to a rooting culture medium; and 1.2) 2-3 plants are placed in each bottle, the bottles are placed in a culture room for 4-d dark culture at a culture temperature of 25 +/- 2 DEG C, then light culture is conducted, fluorescent lamps are used as light source, light intensity is 2,000 lx and light cycle is 16 h; 2) cap-opening domestication is conducted: the rooting-cultured tissueculture seedlings begin to take root in about a week and the rooting of the tissue culture seedlings is completed in about 40 d; and during the domestication, bottle caps are first unscrewed, one daylater, the bottle caps are opened for one third, and three days later, the bottle caps are opened two thirds; 3) hydroponic domestication is conducted: after the cap-opening domestication is conductedfor 7 d, the hydroponic domestication is conducted; and 4) substrate transplantation is conducted: when the seedlings grow to 5 cm or more, the seedlings are transplanted into substrates. The pear tissue culture seedling rooting and domestication method can improve rooting rate and is high in transplanting survival rate.

Description

technical field [0001] The invention relates to the technical field of plant planting, in particular to a method for rooting and domesticating pear tissue cultured seedlings. Background technique [0002] Plant in vitro rapid propagation technology is a commonly used method to create new germplasm. The in vitro rapid propagation of Pyrus plants has been widely used, but there are still many shortcomings: pear is a taproot plant, and it is difficult for tissue cultured seedlings to take root. When transplanting, the tissue-cultured seedlings are directly transferred to the substrate for cultivation after opening the cover and acclimating, and the young root system cannot obtain sufficient nutrition from the substrate, resulting in necrosis of the growth point, making it difficult for the tissue-cultured seedlings of Pyrus to take root even if they are successful. applied to production. Contents of the invention [0003] In order to solve the technical problems in the back...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/04A01G31/00A01G24/15
CPCA01H4/008A01G7/045A01G31/00A01H4/001A01G24/15Y02P60/14
Inventor 徐凌飞温瑶史倩蓉
Owner NORTHWEST A & F UNIV
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