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Tissue culture rapid breeding technology for notopterygium incisum seeds

A seed group and notopterygium technology, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low value-added coefficient, affecting the quality and efficiency of notopterygium tissue culture seedlings, long cultivation cycle, etc.

Inactive Publication Date: 2018-11-02
四川千草生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned prior art scheme, because the root buds or young leaves of notopterygium are used as explants, the tissue induction culture is seriously affected by the low temperature in autumn and winter or the high temperature in summer, and the cultivation period after germination is long and the value-added coefficient is low, thus affecting the quality of notopterygium. Tissue Culture Seedling Quality and Efficiency

Method used

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Experimental program
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Embodiment

[0029] The specific embodiment of the present invention is described in detail in conjunction with embodiment as follows:

[0030] S1. Select the notopterygium seeds that have been treated in layers, soak them in water completely, and peel off the swollen seed coat after 30 minutes. After the seed coat is completely peeled off, the seeds are rinsed under running water for 30 minutes. Spread the treated seeds on the filter paper and transfer to the ultra-clean workbench.

[0031] S2. Pour 70% alcohol into a beaker, soak the seeds in the beaker for 30 seconds, take it out immediately, and rinse with sterile water 2-3 times. After absorbing too much water with filter paper, put the seeds into a flask filled with 0.1% mercury liter solution, soak for 8 minutes, take them out, and rinse them with sterile water for 3 to 5 times. The filter paper absorbs the water on the surface of the seeds.

[0032] S3. Configure MS+NAA1.0mg / l medium as the basic medium. The seeds treated in ste...

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Abstract

The invention discloses a tissue culture rapid breeding technology for notopterygium incisum seeds. The tissue culture rapid breeding technology comprises the following steps: inoculating the notopterygium incisum seeds subjected to stratifcation treatment to a basal culture medium to obtain 50 percent or above of induced germination rate; subsequently, transferring buds to a proliferation culturemedium and enabling a proliferation system to be improved to about 6, wherein plants have no malformation or browning; finally, segmenting a proliferation body into a single plant for rooting culture. According to the tissue culture rapid breeding technology disclosed by the invention, the notopterygium incisum seeds are adopted to replace radical buds or young leaves of notopterygium incisum toserve as explants for tissue culture, and the influence of ambient temperature on explant culture is eliminated; in addition, the proliferation efficiency of the explants is remarkably improved, and thereby rapid breeding of notopterygium incisum seedlings is realized.

Description

technical field [0001] The invention relates to a rapid breeding technology of notopterygium seeds through tissue culture, and belongs to the technical field of notopterygium tissue culture and seedling cultivation. Background technique [0002] Notopterygium is a perennial herb of Umbelliferae notopterygii. It uses rhizomes and roots as medicine. It is warm in nature and bitter in taste. It can not only produce sweat and relieve the exterior, but also dispel rheumatism and relieve pain. Arthralgia, joint pain, bruises and other diseases. It is mainly distributed in alpine forest-shaded wetlands in the alpine regions of Sichuan, Qinghai, Gansu, Tibet and other provinces. The seed reproduction ability is low, the growth is slow, and the distribution range is narrow, resulting in a small number of wild plants. In recent years, with the diversification of uses of notopterygium, its market price has continued to rise, resulting in a large amount of excavation, which has brought...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李强黄作喜陈兴贵
Owner 四川千草生物科技股份有限公司
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