Tissue culture rapid breeding technology for notopterygium incisum seeds
A seed group and notopterygium technology, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low value-added coefficient, affecting the quality and efficiency of notopterygium tissue culture seedlings, long cultivation cycle, etc.
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[0029] The specific embodiment of the present invention is described in detail in conjunction with embodiment as follows:
[0030] S1. Select the notopterygium seeds that have been treated in layers, soak them in water completely, and peel off the swollen seed coat after 30 minutes. After the seed coat is completely peeled off, the seeds are rinsed under running water for 30 minutes. Spread the treated seeds on the filter paper and transfer to the ultra-clean workbench.
[0031] S2. Pour 70% alcohol into a beaker, soak the seeds in the beaker for 30 seconds, take it out immediately, and rinse with sterile water 2-3 times. After absorbing too much water with filter paper, put the seeds into a flask filled with 0.1% mercury liter solution, soak for 8 minutes, take them out, and rinse them with sterile water for 3 to 5 times. The filter paper absorbs the water on the surface of the seeds.
[0032] S3. Configure MS+NAA1.0mg / l medium as the basic medium. The seeds treated in ste...
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