A kind of two-color fluorescent probe and its synthesis method and application

A dual-color fluorescent probe and fluorophore technology is applied in the field of dual-color fluorescent probes and their synthesis, and achieves the effects of simple synthesis, sensitive detection and simple operation.

Active Publication Date: 2020-11-20
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, many detection methods have been developed to detect O 2 ·- or Zn 2+ fluorescent probes, but these probes are used for single-component detection and imaging at the cellular level. Due to the complex biological background of the brain and the existence of the blood-brain barrier, they are used for the simultaneous detection of O in vivo 2 ·- and Zn 2+ probes have not been reported

Method used

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  • A kind of two-color fluorescent probe and its synthesis method and application
  • A kind of two-color fluorescent probe and its synthesis method and application
  • A kind of two-color fluorescent probe and its synthesis method and application

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preparation example Construction

[0033] In another specific embodiment of the present invention, the preparation method of the above-mentioned two-color fluorescent probe is provided, and the preparation method includes the following reactions:

[0034]

[0035] In yet another specific embodiment of the present invention, the step a is specifically:

[0036] Under the protection of an inert gas, bromopropylamine, DPA, and potassium carbonate were mixed and stirred, and purified to obtain a light yellow solid A. a.

[0037] In yet another specific embodiment of the present invention, the step b is specifically:

[0038] Under the protection of an inert gas, mix caffeic acid, DMAP, DCC, triethylamine and DMF, and stir in an ice bath to obtain an activated caffeic acid solution;

[0039]Under the protection of an inert gas, after mixing ethylenediamine and DMF, the activated caffeic acid solution was added dropwise and stirred at room temperature to obtain a brown turbid liquid, which was obtained as a ligh...

Embodiment 1

[0052] A method for detecting O in cells and in vivo 2 ·- and Zn 2+ The novel fluorescent probe, its preparation method is as follows:

[0053]

[0054] Step a: under nitrogen protection conditions, 2.18g bromopropylamine, 1.99gDPA, 0.50g K 2 CO 3 Put 30ml of acetonitrile into a two-necked flask, stir at room temperature for 12h, filter, and rotary evaporate to obtain light yellow solid A. Mass Spectrum See Figure 4A .

[0055]

[0056] Step b: Under the protection of nitrogen, put 1.8g of caffeic acid, 1.2g of DMAP, 2g of DCC, 1.4ml of triethylamine and 12ml of DMF into a two-necked flask, and stir for 30 minutes in an ice bath to obtain an activated caffeic acid solution. Under the condition of nitrogen protection, 670 microliters of ethylenediamine and 5ml DMF were added into the three-necked flask, and then the activated caffeic acid solution was added dropwise into the three-necked flask, and stirred at room temperature for 12 hours to obtain a brown turbid l...

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Abstract

The invention provides a double-color fluorescent probe as well as a synthesis method and application thereof, the double-color fluorescent probe is especially suitable for simultaneously detecting O2. <-> and Zn < 2 + > in a living body, the double-color fluorescent probe is composed of dipyridylmethylamine(DPA), caffeic acid and 4-bromo-1, 8-naphthoic anhydride, the double-color fluorescent probe has the advantages of simple preparation, sensitive detection, good selectivity and the like. According to the method, the change of the O2. <-> and Zn < 2 + > in the brain of the mouse with depression is successfully imaged and analyzed at the living body level for the first time, and the method has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of biological detection and fluorescent probe preparation, and specifically a two-color fluorescent probe and its synthesis method and application. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Depression, as a class of mental illness, has extremely high morbidity and mortality, and poses serious harm to human health. However, the current understanding of the pathophysiology of depression is still far from complete. In order to effectively prevent and treat depression, we must have a thorough understanding of the occurrence and development of depression. Fluorescence imaging technology ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D401/14C09K11/06A61K49/00
CPCA61K49/0021C07D401/14C09K11/06C09K2211/1029
Inventor 唐波丁琪李平王昕
Owner SHANDONG NORMAL UNIV
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