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Bacterium drug sensitivity detection method suitable for drug sensitivity plate

A detection method and bacterial technology, which can be applied in the directions of microorganism-based methods, biochemical equipment and methods, and microorganism determination/inspection, etc., can solve the problems of single variety, cumbersome operation, long time consumption, etc., and achieve easy operation and wide application value. , moderate price effect

Inactive Publication Date: 2020-04-03
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a bacterial drug sensitivity detection method suitable for drug sensitivity panels. The drug sensitivity panel provided by the present invention solves the problem of the single variety of reagents for drug sensitivity detection on the market and the cumbersome operation when detecting a large number of bacterial drug sensitivity. Time-consuming problem; the detection method of the present invention can directly determine the antibacterial effect of the drug on bacteria by reading the absorbance with a microplate reader, and apply the formula to calculate the specific antibacterial rate of the drug on bacteria; the drug-sensitive plate provided by the present invention and The drug sensitivity detection method is easy to operate and moderately priced. It is not only suitable for grass-roots veterinarians to efficiently and accurately select therapeutic drugs, but also can be used for statistics of drug resistance in large batches of samples in scientific research experiments. It has wide application value

Method used

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  • Bacterium drug sensitivity detection method suitable for drug sensitivity plate
  • Bacterium drug sensitivity detection method suitable for drug sensitivity plate
  • Bacterium drug sensitivity detection method suitable for drug sensitivity plate

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Experimental program
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Effect test

Embodiment 1

[0050] A drug sensitivity panel for detecting the susceptibility of bacteria to drugs, the drug sensitivity panel comprising:

[0051] a. The cover of the plate is marked with detection wells, blank control wells, positive control wells and negative control wells; the detection wells are marked with what kind of drug is coated;

[0052] b. Plate body: There are detection holes, blank control holes, positive control holes and negative control holes, and the detection holes are coated with drugs commonly used to treat Gram-positive and Gram-negative bacteria infections. If a large number of samples are detected For the sensitivity of multiple drugs, you can choose a drug-sensitive plate coated with multiple drugs and the drug concentration is the minimum inhibitory concentration (MIC) of the standard strain. Drug susceptibility plate with multiple drugs and drug concentration gradient.

[0053] Taking the drug sensitivity plate prepared by a conventional 96-well plate as an exa...

Embodiment 2

[0070] The preparation method of multi-sample drug-sensitivity plate, the specific steps are as follows:

[0071] ①Preparation of drug solution: according to the concentration marked on the cover of the drug sensitivity plate, use the corresponding solvent to dilute the pure drug powder to a concentration of 100 times, and store it at -20°C as the drug stock solution for configuring the drug sensitivity plate;

[0072] ②Dilute medicinal solution: use carnosol antioxidant coating solution (use absolute ethanol to dissolve carnosol, the concentration of carnosol in the prepared solution is 0.2mg / mL, after the solution is sterilized and filtered, the coated solution is obtained. Dilution) Dilute the drug solution at low temperature (about 16°C) to the concentration indicated on the plate cover of the drug-sensitivity plate, and add it to the detection well of the drug-sensitivity plate body, adding 100 μL of drug solution to each well;

[0073] ③Drying of the drug-sensitive plate...

Embodiment 3

[0076] The preparation method of MIC drug-sensitivity plate, concrete steps are as follows:

[0077] ①Preparation of drug solution: According to the concentration marked on the cover of the drug sensitivity plate, use the corresponding solvent to dilute the pure powder of the drug to 10 times the marked concentration, and use it as the drug stock solution for the drug sensitivity plate, and store it at -20°C;

[0078] ②Dilution of medicinal solution: Use carnosol antioxidant coating solution to dilute the medicinal solution at low temperature (about 16°C) to the concentration indicated on the cover of the drug sensitive plate, and add it to the drug hole in row A of the drug sensitive plate body In each well, add 200 μL of the drug solution, and then use the anti-oxidation coating solution to dilute the drug solution in row A by 2 times to row H;

[0079] ③Drying of the drug-sensitive plate: place the drug-sensitive plate after adding the liquid medicine in a vacuum drying ove...

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Abstract

The invention discloses a bacterial drug sensitivity detection method suitable for a drug sensitivity plate, and belongs to the technical field of bacterial drug sensitivity detection. The drug sensitivity plate disclosed by the invention for detecting drug sensitivity of bacteria is coated with a drug suitable for a bacteria drug sensitivity test. The drug concentration is the minimum inhibitoryconcentration of a standard strain or the gradient concentration taking the minimum inhibitory concentration of the standard strain as the standard. The bacterium drug sensitivity detection method suitable for the drug sensitivity plate comprises the following steps: determining initial bacterium solution absorbance suitable for a drug sensitivity test, and carrying out bacterium drug sensitivitydetection. The drug sensitivity plate and the drug sensitivity detection method provided by the invention are simple and convenient to operate and moderate in price, not only are suitable for high-efficiency and accurate selection of therapeutic drugs by grassroots veterinarians, but also can be used for drug resistance condition statistics of large-batch samples in scientific research tests, andhave wide application value.

Description

technical field [0001] The invention relates to the technical field of bacterial drug sensitivity detection, in particular to a bacterial drug sensitivity detection method suitable for a drug sensitivity plate. Background technique [0002] Bacterial drug resistance is a problem that has existed since humans discovered and widely used antibiotics. Some bacterial strains are naturally resistant to certain antibiotics, and some bacterial strains have produced or acquired drug resistance under external environmental pressure. Extensive drug use will not only lead to the abuse of antibiotics, but will further increase the speed of drug-resistant strains and the multi-drug resistance of strains. Therefore, it is very important to conduct drug susceptibility tests before treating bacterial diseases. [0003] However, the common and commonly used reagents for drug susceptibility testing on the market are few in variety, single in style, cumbersome in operation, and the experimental...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/19C12R1/445C12R1/46
CPCC12Q1/18G01N2333/245G01N2333/31G01N2333/315
Inventor 郭抗抗刘勇李鑫鑫张彦明
Owner NORTHWEST A & F UNIV
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