GABA and electrophysiology micro-nano synchronous sensing detection chip and preparation method thereof

Abstract

The invention provides a GABA and electrophysiological micro-nano synchronous sensing detection chip and a preparation method thereof. The micro-nano synchronous sensing detection chip comprises a substrate layer, a conductive layer and an insulating layer, wherein the substrate layer is made of a flexible material and covers a bottom support; the conductive layer is formed on the substrate layerand used for synchronously detecting changes of inhibitory neurotransmitter GABA and neuroelectric signals in multiple different sub-regions of a rat hippocampus structure; the conductive layer comprises detection site groups which are distributed at positions matching with the size of the rat hippocampus structure and distributed in different sub-regions of the rat hippocampus structure in a radiation manner along the diagonal direction of a detection region; and the insulating layer is made of a flexible material and is formed on the substrate layer except the area where the detection site groups are located. According to the invention, the inhibitory neurotransmitter and the electrical signals can be synchronously detected, and a microprocessing technology, a nanometer material and a biologically sensitive film technology are combined, so the neural information network and the pathogenesis of neurodegenerative diseases can be easily found and understood.

Description

technical field [0001] The disclosure relates to the field of micro-processing technology of biosensors, in particular to a micro-nano synchronous sensing and detection chip for epileptic hippocampal slices of γ-aminobutyric acid and neural electrical activity and a preparation method thereof. Background technique [0002] The brain is composed of a large number of synapses, and the synapses carry out the interactive transduction of nerve signals through the two transmission methods of neurotransmitters and nerve electrical signals to complete our daily behavior, motor learning and other functional activities. Abnormal release of neurotransmitters between synapses or abnormal discharge of electrical signals may indicate the existence of potential neurological diseases. Simultaneously detecting the changes of the two dual-mode signals will help to obtain neurocytopathy in advance, and help to grasp and understand the pathogenic mechanism of neurological diseases and find more...

AI Technical Summary

Problems solved by technology

The detection of GABA neurotransmitter has caused great difficulties in real-time detection in vivo due to its lack of electroactive groups and unique specific enzymes

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