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Bacillus and application thereof in reduction and resourceful production of organic fertilizer from kitchen wastes

A technology for kitchen waste and Bacillus, applied in the field of biological bacterial fertilizer, can solve the problems of bad environmental sanitation, easy growth of various pathogenic microorganisms, etc., and achieve the effects of easy operation, rapid resource utilization and simple equipment

Active Publication Date: 2020-03-27
江苏大道生物环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Food waste is rich in nutrition, high in water content, easy to rot and produce irritating odor, which has a bad impact on environmental sanitation, and is easy to breed various pathogenic microorganisms, mosquitoes and other pests

Method used

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  • Bacillus and application thereof in reduction and resourceful production of organic fertilizer from kitchen wastes
  • Bacillus and application thereof in reduction and resourceful production of organic fertilizer from kitchen wastes
  • Bacillus and application thereof in reduction and resourceful production of organic fertilizer from kitchen wastes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening and Identification of Example 1 Bacterial Strains

[0042] (1) Put 10 g of the collected soil sample near the canteen of the enterprise into a conical flask with 90 ml of glass beads aseptic water, shake it at 200 r / min at 30 ° C for 30 min, put it in a water bath at 80 ° C for 10 min, and then press the gradient Serially diluted and spread on LB plates. The coated LB plate was cultured at 28°C for 3 days, and different single colonies were picked, separated and purified by streaking on the LB plate for 3 times, followed by microscopic examination, and the strain with spores was selected.

[0043] (2) Inoculate the bacillus isolated in step (1) into LB medium respectively, cultivate for 16 hours, and obtain the seed liquid;

[0044] (3) Use the kitchen waste collected from the enterprise canteen as the culture substrate, inoculate 10% of the seed solution in step (2), ferment at 55°C for 48 hours, and detect the changes in the fat content of the fermentation ...

Embodiment 2

[0051] (1) Salmonella typhimurium SL1344 was activated and cultured in LB medium, and shaken at 225 rpm for 12 hours at 37°C.

[0052] (2) After the 4 bacillus strains obtained by screening in Example 1 were activated and subcultured, they were cultured with LB for 14 hours at 37° C. and then centrifuged, and the cultures were collected and used as a control group. The culture supernatant and the control group were filtered through a sterile filter with a pore size of 0.22 microns for use.

[0053] (3) Add 50 μL of bacterial concentration to 5 mL of the culture supernatant of the above-mentioned 4 strains of Bacillus to 1×10 9 CFU / mL Salmonella SL1344 bacteria liquid, cultured at 37°C for 24 hours, when measuring the OD of the co-culture 600 .

[0054] The growth inhibitory effect of table 2 bacillus to salmonella

[0055]

[0056] The results show that Bacillus licheniformis 3 has a good inhibitory effect on Salmonella, and it is preserved as a patented strain.

Embodiment 3

[0057] Example 3 Biological control strains are used for composting of kitchen waste

[0058] (1) Inoculate the Bacillus licheniformis with biological control function obtained by screening in Example 2 into 1 L of LB liquid medium, cultivate it on a shaker at 28° C. and 140 rpm for 2 days, and obtain a bacterial concentration of 10 9 The CFU / mL bacterial liquid was used as the next step of fermentation seed liquid.

[0059] (2) Take 300 kilograms of kitchen waste, pass through a 20-mesh sieve after crushing, and mix with about 150 kilograms of bran after sieving, and the water content after mixing is between 45-50%.

[0060] (3) Put the mixture of food waste and bran in step (2) into a solid-state fermenter developed by Jiangsu Avenue Bioenvironmental Technology Co., Ltd., and inoculate 1 kg of lichen spores in step (1) for every 100 kg of food waste bacillus liquid.

[0061] (4) The mixture of food waste, bran and strains is heated to 80°C in the fermenter, kept for 10 min...

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Abstract

The invention discloses bacillus and an application thereof in reduction and resourceful production of an organic fertilizer from kitchen wastes, belonging to the technical field of biological bacterial fertilizers. According to the invention, a bacillus strain with obvious antagonistic action on fusarium is obtained by screening and is used for composting the kitchen wastes, so rapid reduction and resourceful utilization of the kitchen wastes can be realized. The bacterial fertilizer prepared by using the method provided by the invention can improve the physicochemical and biological characteristics of soil and inhibit the occurrence of plant diseases, and has important industrial application prospects.

Description

technical field [0001] The invention relates to bacillus and its application in the reduction and recycling of kitchen waste to produce organic fertilizer, and belongs to the technical field of biological bacterial fertilizer. Background technique [0002] Food waste is rich in nutrition, high in water content, easy to rot and produce irritating odor, which has a bad impact on environmental sanitation, and is easy to breed various pathogenic microorganisms, mosquitoes and other pests. Because of Chinese eating habits. Table waste is astonishing, and a huge amount of food waste is produced every day. Huge environmental pollution is caused, and there is an urgent need for a fast and efficient reduction treatment method for kitchen waste. [0003] In my country's agricultural production, there is a huge gap in organic fertilizers. Organic fertilizers have comprehensive nutrients and high organic matter content. Long-term application can increase soil organic matter, improve s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60C12R1/10
CPCC05F5/002C05G3/00C12N1/20C12N1/205C12R2001/10C05F9/00
Inventor 殷瞻远张向东殷嘉伟
Owner 江苏大道生物环境科技有限公司
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