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Micro RNA and application thereof in preparation of an anti-tumor drug

A tumor and drug technology, applied in the field of microRNA and its application in the preparation of anti-tumor drugs, can solve problems such as quality of life and quality of life threats, patients' families and social burdens, and achieve inhibition of tumor cell growth and/or proliferation Effect

Pending Publication Date: 2020-03-24
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the forecast of the International Agency for Research on Cancer, if no effective measures are taken, the number of cancer cases in my country will reach 4 million and the death toll will reach 3 million by 2020. The occurrence of tumors poses a serious threat to people's quality of life and quality of life. A heavy burden on patients' families and society

Method used

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  • Micro RNA and application thereof in preparation of an anti-tumor drug
  • Micro RNA and application thereof in preparation of an anti-tumor drug
  • Micro RNA and application thereof in preparation of an anti-tumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The RNA sequence for the experiment is shown in Table 1:

[0063] Table 1 microRNA sequence

[0064]

[0065]

[0066]

[0067] Experimental method of the present invention comprises:

[0068] 1. Cell culture

[0069] All cell lines were cultured in RPMI-1640 or DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 , Saturated humidity. The cells were grown to about 80%, digested and passaged with 0.25% trypsin. Observe the morphology and growth rate of the cells every day, and replace the fresh medium in time.

[0070] 2. MicroRNA transfection

[0071] Press 3×10 3 Cells in the exponential growth phase were inoculated in 96-well plates at an amount of 3 cells / well, and cultured overnight until 80% of the cells were confluent. After 24 hours, 1, 2, 10, 25, 50, 100 nM microRNAs were diluted into 25 μL of RPMI1640 medium without serum and antibiotics, and mixed gently with a pipette; 1 μL of Liposome 2000 (Invitrogen Corporation) ) suspension was...

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Abstract

The invention relates to the technical field of biology, in particular to RNA or application thereof. The length of the RNA molecule provided by the invention is 22 basic groups; the first to sixth base sequences of the 5'end of the RNA molecule are UGGCAG, or the 17th to 22th basic groups are UGCCCCU. The growth and / or proliferation of tumor cells can be remarkably inhibited by using the RNA molecule, the, so that an anti-tumor effect is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to microRNA and its application in the preparation of antitumor drugs. Background technique [0002] Malignant tumors seriously threaten human health and life, and have become the primary cause of death for urban and rural residents in my country. Among them, lung cancer has the highest morbidity and mortality rate, followed by liver cancer, colorectal cancer, and gastric cancer. Relevant statistical data show that in 2015, there were 3.929 million new cases of malignant tumors nationwide, with an incidence rate of 285.83 / 100,000; 2.338 million cases of deaths due to tumor diseases, with a mortality rate of 170.05 / 100,000. According to the forecast of the International Agency for Research on Cancer, if no effective measures are taken, the number of cancer cases in my country will reach 4 million and the death toll will reach 3 million by 2020. The occurrence of tumors poses a serious t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61P35/00A61P35/02
CPCC12N15/1135A61P35/00A61P35/02C12N2310/141
Inventor 汪维鹏孔洁红孟凡义
Owner SUZHOU UNIV
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