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Method for cellulase assisted ultrasonic wave extraction of red-pulp pitaya pigment

A red-flesh dragon fruit and auxiliary extraction technology, which is applied to chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of reducing time-consuming, reducing the residual rate of dragon fruit pigment solvent, and low dissolution rate of dragon fruit pigment. , to achieve the effect of improving extraction yield and benefiting retention

Active Publication Date: 2020-03-24
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that cellulase cooperates ultrasonic assisted extraction red meat pitaya pigment, to solve the problem that the extracting efficiency is reduced because of the low dissolution rate of pitaya pigment in the prior art and time-consuming, and reduces Solvent residual rate in pitaya pigment

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  • Method for cellulase assisted ultrasonic wave extraction of red-pulp pitaya pigment
  • Method for cellulase assisted ultrasonic wave extraction of red-pulp pitaya pigment
  • Method for cellulase assisted ultrasonic wave extraction of red-pulp pitaya pigment

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Embodiment Construction

[0034] The following is further described in detail through specific implementation methods:

[0035] In the present invention, the extraction of pigments is carried out according to the following steps, a method for extracting red meat dragon fruit pigments with the aid of cellulase and ultrasonic waves, comprising the following steps:

[0036] (1) Get undamaged dragon fruit to clean, remove surface scales, and dry;

[0037] (2) the peel and pulp of dragon fruit in step (1) are separated, the peel is cut into strips, and the pulp is beaten;

[0038] (3) take the pericarp or pulp in step (2) and freeze-dry, crush and sieve the freeze-dried pericarp or pulp to obtain powder;

[0039] (4) For the powder in step (3), add extractant and auxiliary enzyme, the mass ratio of pericarp or pulp to the extract is 1:50-300; the extract used in this embodiment is distilled water, ethanol, acetone and / or or methanol; the auxiliary enzyme is pectinase or cellulase; use ultrasonic treatment...

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Abstract

The invention belongs to the technical field of pigment extraction, and particularly discloses a method for cellulase assisted ultrasonic wave extraction of red-pulp pitaya pigment. The method comprises the following steps: (1) cleaning undamaged pitaya, removing scales on the surface, and airing; (2) separating the peels and the pulps of the pitaya in the step (1); (3) freeze-drying the peels orthe pulps obtained in the step (2), crushing the freeze-dried peels or pulps, and screening with a 40 mesh sieve to obtain powder; (4) adding an extracting agent and an auxiliary enzyme into the powder obtained in the step (3), extracting a pigment by using ultrasonic treatment, performing centrifugal separation, and taking the supernatant; and (5) carrying out reduced pressure rotary evaporationon the supernatant obtained in the step (4) to remove the solvent, and concentrating to obtain a red pigment crude extraction liquid. The method is mainly used for extracting the pitaya pigment, the problems of low dissolution rate of the pitaya pigment and extraction efficiency reducing caused by long time consumption in the existing pitaya pigment extraction technology are solved, and the solvent residual rate in the pitaya pigment is reduced.

Description

technical field [0001] The invention belongs to the technical field of pigment extraction, and specifically discloses a method for extracting red flesh pitaya pigment with the assistance of cellulase and ultrasonic waves. Background technique [0002] Dragon fruit belongs to the cactus family. It is rich in vegetable protein and dietary fiber. Regular consumption can improve constipation. It can also be used as an adjuvant therapy for diabetics and hyperlipidemia patients. Pitaya's peel and flesh are bright in color, rich in betaine pigments, and it is an excellent resource for preparing natural pigments. [0003] At present, the pitaya pigment at home and abroad mainly adopts the solvent extraction method, and the obtained dragon fruit pigment product is not high in purity, takes a long time, and reduces the extraction efficiency; at the same time, there are extractant residues in the pitaya pigment product, and the pigment product contains Problems such as pectin make the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 邓放明田艳段晓嫣
Owner HUNAN AGRICULTURAL UNIV
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