Carassius auratus hypoxia-inducible factor HIF-1alpha gene, cloning method and application

A technology of hypoxia-inducible factor, HIF-1, applied in the field of molecular biology, can solve the problem of undiscovered patent publications and other issues

Pending Publication Date: 2020-03-20
天津市水产研究所
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Carassius auratus hypoxia-inducible factor HIF-1alpha gene, cloning method and application
  • Carassius auratus hypoxia-inducible factor HIF-1alpha gene, cloning method and application
  • Carassius auratus hypoxia-inducible factor HIF-1alpha gene, cloning method and application

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Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0031] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0032] A goldfish hypoxia-inducible factor HIF-1α gene, the nucleotide sequence of the goldfish hypoxia-inducible factor HIF-1α gene is SEQ ID NO.1.

[0033] As for the amino acid encoded by the goldfish hypoxia-inducible factor HIF-1α gene, the amino acid sequence is SEQ ID NO.2.

[0034] The cloning method of the goldfish hypoxia-inducible factor HIF-1α gene as described above, the steps are as follows:

[0035] Step 1: Extract total RNA from goldfish liver, gills, brain, and heart tissues, and reverse transcribe t...

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Abstract

The invention relates to a carassius auratus hypoxia-inducible factor HIF-1alpha gene. The carassius auratus hypoxia-inducible factor HIF-1alpha gene has a nucleotide sequence of SEQ ID NO. 1. The invention discloses a nucleotide sequence and an amino acid sequence of the carassius auratus hypoxia-inducible factor HIF-1alpha gene for the first time; the carassius auratus hypoxia-inducible factor HIF-1alpha gene is verified to exert effects in hypoxic stress of carassius auratus through a qPCR technology; the expression mode of carassius auratus hypoxia-inducible factor HIF-1alpha in liver, brain, heart and gill tissues is analyzed by utilizing real-time fluorescence quantitative qPCR; and results show that the expression modes of HIF-1alpha genes in the liver, brain, heart and gill tissuesof the carassius auratus are different under the condition of hypoxia stress, and basic data and a reference can be provided for a fish hypoxia adaptation mechanism, a hypoxia signal transduction path and cultivation of novel hypoxia-resistant fish varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to a goldfish hypoxia-inducible factor HIF-1α gene, a cloning method and application thereof. Background technique [0002] Oxygen is essential to most living things. In general, humans and other mammals have poor oxygen tolerance, and prolonged hypoxia may cause tissue failure or even death. However, marine invertebrates and fish have a strong ability to tolerate low oxygen, and can survive under low oxygen or even anaerobic conditions for a period of time. In response to hypoxic environments, these organisms can regulate physiological processes such as glucose transport, glycolysis, and red blood cell production by controlling the expression of related genes, thereby enhancing the body's tolerance to hypoxia. [0003] Hypoxia-inducible factor-1 (hypoxia-inducible factor, HIF-1) is an important transcription factor that exists in various animals and humans. It can regul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/705C12N15/12C12N15/10C12Q1/6851
CPCC07K14/70567C12Q1/6851
Inventor 李春艳姜巨峰刘克明孟一耕蔡超刘肖莲白晓慧
Owner 天津市水产研究所
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