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Biological microbial inoculum for improving micro-ecological environment level of tobacco planting soil

A biological inoculum and micro-ecological technology, applied in the field of biological inoculum formulation, can solve the problems of low organic matter content, soil acidification, and inability to effectively and efficiently change the soil, and achieve the effects of improving the soil environment, improving acidification, and facilitating the formation of humus.

Active Publication Date: 2020-03-17
LINCANG OF YUNNAN TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the soil where tobacco plants are planted continuously all year round, problems such as soil acidification and low organic matter content are prone to occur; existing biological bacteria agents cannot effectively and efficiently change the above-mentioned problems existing in the soil

Method used

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  • Biological microbial inoculum for improving micro-ecological environment level of tobacco planting soil
  • Biological microbial inoculum for improving micro-ecological environment level of tobacco planting soil
  • Biological microbial inoculum for improving micro-ecological environment level of tobacco planting soil

Examples

Experimental program
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Effect test

Embodiment 1～5

[0041] The preparation of embodiment 1~5 biological bacteria agent 1~5

[0042] The bacterial strains of Bacillus amyloliquefaciens, Pseudomonas fluorescens, and Bacillus pumilus make the biocontrol bacteria agent according to the following steps respectively:

[0043] a. Transplant the strain into a test tube containing LB medium for one-ring activation, and culture at 32°C for 48 hours;

[0044] The formula of LB liquid medium is: tryptone (Tryptone) 10g / L, yeast extract (Yeasextract) 5g / L, sodium chloride (NaCl) 5g / L, pH7.0;

[0045] b. Get a ring of activated bacterial lawn and move it into the LB culture solution described in 600ml of 1000ml Erlenmeyer flask, cultivate it for 72 hours at 120 rpm and 32°C to obtain the fermentation broth;

[0046] c. Inoculate the fermentation liquid into the LB culture liquid at a ratio of 1:200 times of the inoculum, and carry out fermentation culture in the seed tank. The temperature of the seed tank is 32°C. Fermentation seed strains ...

Embodiment 6

[0051] The soil experiment of embodiment 6 biological bacteria agent 1~3

[0052] The composite microbial bacterial agent produced by the method of using embodiments 1~3 carries out field test, test site: the planting area of ​​Yunyan 87 flue-cured tobacco in Mengsa Town, Gengma County, Lincang City, Yunnan Province (99 ° 23 '22 "E, 23°33'40"N).

[0053] The planted species in the test is flue-cured tobacco, and the experimental field for the test is a continuous monoculture of flue-cured tobacco for 12 years, the average soil pH value is 5.30, the soil organic matter content is higher than 30g / kg, and the plot with a high incidence of flue-cured tobacco is spread with organic fertilizer.

[0054] Four treatments were set up in the experiment, namely:

[0055] 1, embodiment 1 gained inoculum 1 (50 times of liquid dips in root / irrigates root);

[0056] 2. The bacterial agent 2 obtained in embodiment 2 (50 times of liquid is dipped in root / irrigated root);

[0057] 3. The bac...

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Abstract

The invention discloses a biological microbial inoculum for improving the micro-ecological environment level of tobacco planting soil. The biological microbial inoculum comprises bacillus amyloliquefaciens, pseudomonas fluorescens and bacillus pumilus, wherein the bacillus amyloliquefaciens is a strain with a preservation number of CGMCC No. 6598. The microbial inoculum can improve acidification and organic matter content of long-term tobacco planting soil, and at the same time can play a role in efficiently improving the soil environment.

Description

technical field [0001] The application relates to a biological agent for improving the micro-ecological environment level of tobacco planting soil, which belongs to the field of biological agent formulation. Background technique [0002] If a single species of plant is planted for a period of time, the soil is prone to a series of problems such as acidification, nutritional imbalance, microbial imbalance, decreased water and fertilizer retention capacity, and serious soil-borne diseases caused by continuous cropping obstacles. How to eliminate the above-mentioned problems and ensure the stability and repeatability of soil use is a technical problem that needs to be solved. [0003] For the soil where tobacco plants are continuously planted all year round, problems such as soil acidification and low organic matter content are prone to occur; existing biological bacteria agents cannot effectively and efficiently change the above-mentioned problems existing in the soil. Conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/39
CPCC12N1/20C12R2001/39C12N1/205C12R2001/07
Inventor 何元胜曹敬东吴巨广孔广才王继明郑元仙许银莲何川赵俊和明东杨国恩杨敏刘佳妮钟宇王启宇方宇余磊
Owner LINCANG OF YUNNAN TOBACCO
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