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Bacillus licheniformis mes816 and its products and applications

A technology of Bacillus licheniformis and MES816, which is applied in the field of microorganisms, can solve the problems that Bacillus licheniformis can be used to prevent and control cotton blight, and achieve the effect of preventing cotton blight, low cost, and promoting production

Active Publication Date: 2021-06-18
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of microorganisms to control soil-borne diseases of crops is one of the most effective control measures. After searching, there is no report on the use of Bacillus licheniformis (B.licheniformis) to control cotton blight

Method used

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  • Bacillus licheniformis mes816 and its products and applications
  • Bacillus licheniformis mes816 and its products and applications
  • Bacillus licheniformis mes816 and its products and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The isolation and identification of embodiment 1 bacterial strain

[0040] The soil in Baoding, Hebei Province was serially diluted with sterile water, cultured at 37°C for 12 hours by streaking on the plate, and a single colony was picked to make a slant strain, and refrigerated at 4°C. A single colony was picked, stained with crystal violet, and examined under a microscope. It was identified as Bacillus licheniformis by the strain detection agency of the Ministry of Agriculture.

[0041] After morphological observation, microscopic examination, enzymatic analysis and carbohydrate utilization experiments, combined with 16S rDNA sequencing comparison, it was identified as Bacillus licheniformis and named MES816. Bacillus licheniformis MES816 is a Gram-positive bacterium that can grow under anaerobic conditions. Bacteria are straight rods, arranged singly, in pairs or in short chains. There are spores, the spores are nearly oval, middle-growing, and the sporangia are n...

Embodiment 2

[0046] The preparation of embodiment 2 microbial bacterial agents

[0047] (1) Shake flask culture: the strain activated on the slant was subjected to seed shake flask culture.

[0048] Shake flask medium is: beef extract peptone medium (beef extract 0.5%-3%, peptone 0.5%-3%, yeast extract 0.2%-2%, sodium chloride 0.3-0.5%, medium pH 6.0-7.5 ).

[0049] The culture conditions are: rotation speed 180-230r / min, temperature 32-37°C, culture for 10-12h.

[0050] (2) Primary seed culture: 100mL primary seed culture medium is placed in a 500mL eggplant bottle, sterilized by moist heat at 116°C-121°C for 20-35min, and 1mL live bacteria of Bacillus licheniformis in a freeze-dried tube is used to inoculate the primary seed In culture medium, culture at 30°C-37°C for 10-12h.

[0051] The raw materials and dosage of the medium used for primary seed cultivation are: beef extract 0.5%-3%, peptone 0.5%-3%, yeast extract 0.2%-2%, sodium chloride 0.3-0.5%, nutrient agar 1.5%-2.0 %, the pH...

Embodiment 3

[0059] Example 3 Antagonism test of Bacillus licheniformis MES816 to Rhizoctonia solani

[0060] (1) Source of cotton blight pathogen: The cotton blight was collected from the cotton blight strain in Gaoyang County, Baoding City, Hebei Province. It was isolated and purified by the company's researchers and identified as Rhizoctonia solani Kohn. The determination of pathogenicity showed strong pathogenicity.

[0061] (2) Tablet confrontation experiment:

[0062] First inoculate the cotton blight on the center of the nutrient agar medium plate, then dip a small amount of activated Bacillus licheniformis MES816 and inoculate it at about 2 cm on both sides of the pathogen, and set only the cotton blight inoculated as the blank control. Cultivate at a constant temperature of 30°C. When the blank control is about to cover the entire petri dish, measure the control growth amount (colony radius) and treatment growth amount (inhibition growth radius after inoculation of MES816) of the...

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Abstract

The invention discloses a bacillus licheniformis MES816 and its products and applications. The present invention is isolated from a strain of Bacillus licheniformis obtained from soil in Baoding, Hebei ( Bacillus licheniformis ) MES816, the strain against the pathogenic bacteria of cotton blight Rhizoctonia solani Kohn It has a strong inhibitory effect, prevents the occurrence of cotton blight, and promotes the increase of cotton production and income.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to bacillus licheniformis MES816 and its products and applications. Background technique [0002] Cotton blight, also known as root rot and black root disease, is the main disease of cotton seedlings, and mostly occurs from seedlings unearthed to true leaves. Cotton seedlings die within a week or so of being infected. It occurs in every major cotton area of ​​our country, and can appear in the field every year. The incidence rate is generally 5%-40%, often resulting in lack of seedlings and broken ridges. In the year of reoccurrence, the incidence rate in some areas can reach more than 50%, which can cause the death of cotton seedlings in pieces, and even cause the destruction of species. [0003] The pathogen of cotton blight is Thanate.pephorus cucumeris (Frank) Donk in the form of Thanate. Known as Rhizoctonia spp. Its active state is only produced under the conditions of high t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/10
CPCA01N63/00C12N1/205C12R2001/10
Inventor 肖培英顾欣燕闫辉敏赵晓琛刘刚
Owner 天津开发区坤禾生物技术有限公司
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