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A kind of rooting method of medicinal plant antler grass tissue culture seedling outside the bottle

A technology of medicinal plants and exogenous roots, which is applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of immature tissue culture and rapid propagation technology, and achieve shortening of the cultivation period, protection of the environment, and simple operation Effect

Active Publication Date: 2022-02-01
EXPERIMENTAL CENT OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the research and practice on the tissue culture of velvet antler shows that its tissue culture and rapid propagation technology is still immature, and a large number of aerial roots distributed on the surface of the medium are induced in the bottle, and there has been no report of transplanting and surviving.

Method used

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  • A kind of rooting method of medicinal plant antler grass tissue culture seedling outside the bottle
  • A kind of rooting method of medicinal plant antler grass tissue culture seedling outside the bottle
  • A kind of rooting method of medicinal plant antler grass tissue culture seedling outside the bottle

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Experimental program
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Effect test

Embodiment 1

[0054] (1) Selection of bottle seedlings: choose cluster buds of velvet antler tissue cultured seedlings that grow well, the leaves are covered with wool, and the color is silvery white ( figure 1 ), for strong seedling cultivation;

[0055] (2) Cultivation of strong seedlings: Cut off the cluster buds of tissue cultured seedlings by individual plants, select single buds with terminal buds and seedling height of 2cm, and transfer them to the strong seedling medium. The strong seedling medium contains the following components: macroelements: KNO 3 :1200mg / L; NH 4 NO 3 :1650mg / L; Ca(NO 3 ) 2 4H 2 O:400mg / L; KH 2 PO 4 :170mg / L; MgSO 4 ·7H 2 O:370mg / L; CaCl 2 ·7H 2 O:240mg / L;

[0056] Organic ingredients: inositol: 200mg / L; glycine: 2mg / L; thiamine hydrochloride: 0.4mg / L; pyridoxine hydrochloride: 0.5mg / L; niacin: 0.5mg / L; other ingredients are the same as MS;

[0057] The medium formula is: improved MS + BA0.3mg / L + IAA0.5mg / L + GA0.2mg / L + sucrose 30g / L + agaric 1.8g...

Embodiment 2

[0063] (1) Selection of bottle seedlings: choose cluster buds of velvet antler tissue cultured seedlings that grow well, the leaves are covered with wool, and the color is silvery white ( figure 1 ), for strong seedling cultivation;

[0064] (2) Cultivation of strong seedlings: Cut off the cluster buds of tissue cultured seedlings by individual plants, select single buds with terminal buds and seedling height of 2cm, and transfer them to the strong seedling medium. The strong seedling medium contains the following components: macroelements: KNO 3 :1200mg / L; NH 4 NO 3 :1650mg / L; Ca(NO 3 ) 2 4H 2 O:400mg / L; KH 2 PO 4 :170mg / L; MgSO 4 ·7H 2 O:370mg / L; CaCl 2 ·7H 2 O:240mg / L;

[0065] Organic ingredients: inositol: 200mg / L; glycine: 2mg / L; thiamine hydrochloride: 0.4mg / L; pyridoxine hydrochloride: 0.5mg / L; niacin: 0.5mg / L; other ingredients are the same as MS;

[0066] The medium formula is: improved MS + BA0.3mg / L + IAA1.0mg / L + GA0.4mg / L + sucrose 30g / L + agaric 2.0g...

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Abstract

The invention belongs to the technical field of herbal medicinal plant tissue culture, and specifically relates to a method for rooting out of a bottle of velvet antler tissue cultured seedlings, which mainly solves the problems of long rooting period in a bottle of tissue cultured seedlings, induction of many aerial roots, and low survival rate of transplanting. The technical problem includes the following steps: (1) selection of bottle seedlings; (2) cultivation of strong seedlings; (3) hardening of seedlings; (4) transplanting of tissue culture seedlings; (5) post-planting management. The rooting technology of grass tissue culture seedlings outside the bottle optimizes its tissue culture and rapid propagation system. This technology does not need rooting in a bottle, and it is directly transplanted after hormone treatment. After two weeks, new roots can be seen from the base of the seedlings. After 30 days, the number of roots will reach 5-10, and the root length will be 0.5-1.5cm. It will take about 2 months. The robust rooted seedlings of pilose antler grass with a seedling height of about 6-10 cm were obtained. Compared with the traditional rooting in a bottle, the period of seedling cultivation is shortened by one month, and the survival rate of transplanting is higher than 85%. By adopting the technology of the invention, the quality of rooted seedlings is significantly improved, and industrial production can be carried out.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture of velvet velvet, in particular to a method for rooting out of a bottle of the tissue culture seedling of the medicinal plant velvet velvet. Background technique [0002] Antler grass is a perennial semi-parasitic small herb of the family Scrophulariaceae, and it is one of the main components of Yanning Granules, a protected variety of traditional Chinese medicine in my country. It is mainly distributed in Jiangxi, Hunan, Fujian, Zhejiang, Jiangsu and other places in southeastern my country. It grows in the weeds on the sunny side of the hillside or under the masson pine forest. It has been reported in the literature that velvet antler contains a variety of secondary metabolites, and the isolated phenylethanol glycosides and flavonoids all have certain anti-complement activities, have antibacterial, anti-inflammatory and anti-viral effects, and are commonly used clinically. It is an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李峰卿曾满生刘素贞
Owner EXPERIMENTAL CENT OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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