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Anti-human claudin 18.2 monoclonal antibody and application thereof

A monoclonal antibody, antibody technology, applied in the field of biomedicine, can solve the problems of reduced expression, no antibody yet, reduced expression of claudin-18, etc.

Pending Publication Date: 2020-03-03
REYOUNG SUZHOU BIOLOGY SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In 2006, Sanada et al. found that the expression of claudin-18 gene was down-regulated in 57% of gastric cancers. Through immunohistochemical analysis, claudin-18 was expressed on the membrane of normal gastric mucosa and duodenal Paneth cells, but in some intestinal carcinomas. In 90% of gastric adenomas, the expression of claudin-18 is reduced, and the expression of claudin-18 is more common in intestinal type gastric cancer than in other types of gastric cancer. It is speculated that it may be involved in the occurrence of early gastric cancer
However, there is no antibody developed to target this protein

Method used

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  • Anti-human claudin 18.2 monoclonal antibody and application thereof
  • Anti-human claudin 18.2 monoclonal antibody and application thereof
  • Anti-human claudin 18.2 monoclonal antibody and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0092] 1. Antigen Preparation

[0093] GST-ECD protein preparation

[0094] The partial sequence of the first extracellular domain of human claudin 18.2 was constructed on the pGEX-KG vector (antigen sequence 1), the constructed expression vector was transformed into E. coli TG1 strain, cultured in LB medium and induced by IPTG expression of antigenic proteins. The collected E. coli TG1 cells were crushed with a homogenizer, affinity purified with GlutathioneSepharose 4 Fast Flow (GE, Cat# 17-5132-01), and then purified with HiTrap Capto Q (GE, Cat# 11001302) ) column for secondary purification and dialyzed the protein into PBS, pH 7.4 to obtain the antigenic protein with a purity of >85%.

[0095] DNA immunogen preparation

[0096] The partial or full-length sequence of the first extracellular domain of claudin 18.2 was constructed on the pcDNA3.1 vector as an immunogen (antigen sequence 2, antigen sequence 3).

[0097] Human claudin 18.1 and human claudin 18.2 stable cel...

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Abstract

The invention relates to the field of biomedicine, in particular to an anti-human claudin 18.2 monoclonal antibody and application thereof. The antibody has three heavy chain complementary determiningregions (heavy chain CDR1, heavy chain CDR2, heavy chain CDR3) and three light chain complementary determining regions (light chain CDR1, light chain CDR2, light chain CDR3), and has good specificity, high affinity and stability.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an anti-human claudin18.2 monoclonal antibody and its application. Background technique [0002] The claudins family is the skeleton protein of tight junctions between epithelial cells, which is involved in the formation of tight junctions. Tight junctions maintain cell polarity and barrier function. The abnormal expression of claudins leads to the destruction of tight junction structure and function, which is the basis of many diseases. Studies have shown that the Claudins family is abnormally expressed in tumors of various epithelial origins, and is closely related to the occurrence and development of these tumors. It has broad application prospects in diagnosis, treatment, and prognosis. Therefore, the Claudins family has become a new research topic in tumor research. hotspot. [0003] The human Claudin18 gene has two different first exons, so two isoforms, Claudin18.1 and Claudin1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N15/13A61K39/395A61P35/00A61P9/00A61P29/00G01N33/68G01N33/577G01N33/574
CPCC07K16/18A61P35/00A61P9/00A61P29/00G01N33/6893G01N33/577G01N33/57484C07K2317/24C07K2317/92C07K2317/732C07K2317/734A61K2039/505G01N2333/47G01N2800/32G01N2800/7095C07K16/28C07K2317/53C07K2317/33C07K2317/34
Inventor 吴燕燕崔文俊胡琛霏
Owner REYOUNG SUZHOU BIOLOGY SCI & TECH CO LTD
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